05-644

Anti-CAR Antibody, clone RmcB

Name: Anti-CAR Antibody, clone RmcB
Brand: MM
Price: 637 CAD

clone RmcB, Upstate^®, from mouse

Synonym(s):

46 kD coxsackievirus and adenovirus receptor (CAR) protein, CVB3 binding protein, CVB3-binding protein, Coxsackievirus B-adenovirus receptor, coxsackie virus B receptor, coxsackie virus and adenovirus receptor

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200 μG

$637.00

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RmcB, monoclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Upstate^®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001338

UniProt accession no.

P78310

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CXADR(1525)

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This Item	FCMAB418PE-I	MABS1271	HPA003342
Sigma-Aldrich 05-644 Anti-CAR Antibody, clone RmcB	Sigma-Aldrich FCMAB418PE-I Milli-Mark^® Anti-CAR-PE Antibody, clone RmcB	Sigma-Aldrich MABS1271 Anti-EPCR Antibody, clone JRK1535	Sigma-Aldrich HPA003342 Anti-CXADR antibody produced in rabbit
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
clone RmcB, monoclonal	clone RmcB, monoclonal	clone JRK1535, monoclonal	clone polyclonal
species reactivity human, rat, mouse	species reactivity human	species reactivity human	species reactivity human
biological source mouse	biological source mouse	biological source mouse	biological source rabbit
technique(s) immunocytochemistry: suitable, western blot: suitable	technique(s) flow cytometry: suitable	technique(s) flow cytometry: suitable, neutralization: suitable	technique(s) immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:200-1:500
Gene Information human ... CXADR(1525)	Gene Information human ... CXADR(1525)	Gene Information human ... PROCR(10544)	Gene Information human ... CXADR(1525)

General description

The coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR′s biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.

~46 kDa

Immunogen

Native human coxsackie virus-adenovirus receptor protein.

Application

Anti-CAR Antibody, clone RmcB detects level of CAR & has been published & validated for use in IC & WB.

Immunocytochemistry:
1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.

Biochem/physiol Actions

Recognizes CAR at MW ~46 kDa.

Physical form

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide, pH 7.4.

Analysis Note

Evaluated by Western Blot on mouse small intestine lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adenovirus serotype 5 infects human dendritic cells via a coxsackievirus-adenovirus receptor-independent receptor pathway mediated by lactoferrin and DC-SIGN.

William C Adams et al.

The Journal of general virology, 90(Pt 7), 1600-1610 (2009-03-14)

The coxsackievirus-adenovirus receptor (CAR) is the described primary receptor for adenovirus serotype 5 (Ad5), a common human pathogen that has been exploited as a viral vector for gene therapy and vaccination. This study showed that monocytes and dendritic cells (DCs)

Vaccination with adenovirus serotypes 35, 26, and 48 elicits higher levels of innate cytokine responses than adenovirus serotype 5 in rhesus monkeys.

Jeffrey E Teigler et al.

Journal of virology, 86(18), 9590-9598 (2012-07-13)

Adenovirus (Ad) vaccine vectors have proven highly immunogenic in multiple experimental models, but the innate immune responses induced by these vectors remain poorly characterized. Here we report innate cytokine responses to 5 different Ad vectors in 26 rhesus monkeys. Vaccination

A monoclonal antibody specific for the cellular receptor for the group B coxsackieviruses.

Hsu KH, Lonberg-Holm K, Alstein B, Crowell RL.

Journal of virology, 62, 1647-1652 (1988)

Defining a Novel Role for the Coxsackievirus and Adenovirus Receptor in Human Adenovirus Serotype 5 Transduction In Vitro in the Presence of Mouse Serum.

Estrella Lopez-Gordo et al.

Journal of virology, 91(12) (2017-04-07)

Human adenoviral serotype 5 (HAdV-5) vectors have predominantly hepatic tropism when delivered intravascularly, resulting in immune activation and toxicity. Coagulation factor X (FX) binding to HAdV-5 mediates liver transduction and provides protection from virion neutralization in mice. FX is dispensable

Adenovirus-induced thrombocytopenia: the role of von Willebrand factor and P-selectin in mediating accelerated platelet clearance.

Othman, M; Labelle, A; Mazzetti, I; Elbatarny, HS; Lillicrap, D

Blood null

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