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MABF2814-100UL Anti-CCR9 Antibody, clone 9B-1

Anti-CCR9, clone 9B-1, Cat. No. MABF2814, is a rat monoclonal antibody that detects CCR9 and is tested for use in Flow Cytometry, Immunohistochemistry, Inhibition Assay, and Neutralizing Activity.

MABF2814-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2814-100UL
DescriptionAnti-CCR9 Antibody, clone 9B-1
Alternate Names
  • C-C chemokine receptor type 9
  • C-C CKR-9
  • CC-CKR-9
  • CCR-9
  • Chemokine C-C receptor 10
  • CDw199
Background InformationC-C chemokine receptor type 9 (UniProt: Q9WUT7; also known as C-C CKR-9, CC-CKR-9, CCR-9, Chemokine C-C receptor 10, CDw199) is encoded by the Crr9 (also known as Cmkbr10) gene (Gene ID: 12769) in murine species. CCR9 is a multi-pass membrane protein of the G-protein coupled receptor 1 family. It has four extracellular domains, seven transmembrane domains, and four cytoplasmic domains and serves as a receptor for chemokine CCL25/TECK. It is highly expressed in the thymus tissue. It is also expressed on all small intestinal T cells and IgA-producing plasma cells (PCs) from the spleen. It is reported to be crucial for the positioning of PCs to the small intestine. Higher expression of CCR9 is observed in newly generated PCs and it is down-regulated once these cells reach their final destination in the intestine. In wild-type mice, a majority of the PCs are shown to locate to the lower half of the villus where CCL25 is also expressed at high levels. CCR9 deficient mice exhibit reduced number of PCs in the intestine and are unable to mount a T cell dependent antigen specific IgA response. CCR9-/- hematopoietic stem cells are deficient in their ability to generate all major thymocyte subsets. Clone 9B-1 is shown to specifically neutralize CCR9 function and inhibits CCL25-induced calcium mobilization in L.1.2 cells. (Ref.: Svensson, M., et al. (2008). J. Leukoc. Biol. 83(1); 156-164; Rivera-Nieves, J., et al. (2006). Gastroenterology. 131(5); 1518-1529; Pabst, O., et al. (2004). J. Exp. Med. 199(3); 411-416).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-CCR9, clone 9B-1, Cat. No. MABF2814, is a rat monoclonal antibody that detects CCR9 and is tested for use in Flow Cytometry, Immunohistochemistry, Inhibition Assay, and Neutralizing Activity.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Neutralizing
  • Inhibition
Application NotesTested applications

Immunohistochemistry Applications: A representative lot detected CCR9 in Immunohistochemistry applications (Rivera-Nieves, J., et al. (2006). Gastroenterology. 131(5):1518-29).

Flow Cytometry Analysis: A representative lot detected CCR9 in Flow Cytometry applications (Rivera-Nieves, J., et al. (2006). Gastroenterology. 131(5):1518-29).

Neutralizing: A representative lot specifically neutralized CCR9 function in L1.2 cells overexpressing CCR9. (Rivera-Nieves, J., et al. (2006). Gastroenterology. 131(5):1518-29).

Inhibition: A representative lot inhibited CCL25-induced calcium mobilization in L1.2-CCR9 cells. (Rivera-Nieves, J., et al. (2006). Gastroenterology. 131(5):1518-29).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of mouse C-C chemokine receptor type 9 (CCR9).
EpitopeN-terminal
Clone9B-1
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 9B-1 is a rat monoclonal antibody that detects C-C chemokine receptor type 9 (CCR9). It targets an epitope within 25 amino acids from the N-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ccr9
Purification MethodProtein G purified
UniProt Number
Molecular Weight41.91 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in L1.2 cells transfected with mouse CCR9.

Flow Cytometry Analysis: A 1:500 dilution of this antibody detected CCR9 in one million L1.2 cells transfected with mouse CCR9.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF2814-100UL 04065270172600