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217640 Anti-CD11a Mouse mAb (38)

Anti-CD11a, mouse monoclonal, clone 38, recognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 cells. It is validated for FC, IP, IHC on frozen sections and for cell inhibition assays.

Anti-CD11a Mouse mAb (38) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-αL Integrin, Anti-LFA-1

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217640
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
217640-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~180 kDa I domain of the αL integrin subunit in Molt-4 human tumor cells.
Catalogue Number217640
Brand Family Calbiochem®
SynonymsAnti-αL Integrin, Anti-LFA-1
References
ReferencesHolness, C.L., et al. 1995. J. Biol. Chem. 270, 877.
Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.
Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Negative controlNalm-6 cells
Positive controlMolt-4 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Cell Inhibition Assays
Flow Cytometry
Frozen Sections
Immunoprecipitation
Application NotesCell Inhibition Assays (1-5 µg/ml, see comments)
Flow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Application CommentsAlso blocks binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured Molt-4 human tumor cells using anti-CD11a antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman monocytes
ImmunogenHuman
Clone38
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
217640-100UG 04055977202922

Documentation

Anti-CD11a Mouse mAb (38) SDS

Title

Safety Data Sheet (SDS) 

Anti-CD11a Mouse mAb (38) Certificates of Analysis

TitleLot Number
217640

References

Reference overview
Holness, C.L., et al. 1995. J. Biol. Chem. 270, 877.
Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.
Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
SynonymsAnti-αL Integrin, Anti-LFA-1
ApplicationCell Inhibition Assays (1-5 µg/ml, see comments)
Flow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
DescriptionProtein A purified mouse monoclonal antibody. Recognizes the I domain of the ~180 kDa integrin α L subunit (CD11a).
BackgroundCD11a (αL integrin) complexes with CD18 (β2 integrin) to form the LFA-1 heterodimer adhesion molecule which binds to three ICAM's 1,2, and 3, also designated CD54, CD102, and CD50, respectively. LFA-1 is expressed on lymphocytes, monocytes, and neutrophils and plays a role in cell-cell adhesion.
HostMouse
Immunogen speciesHuman
Immunogenhuman monocytes
Clone38
IsotypeIgG2a
Specieshuman
Positive controlMolt-4 cells
Negative controlNalm-6 cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAlso blocks binding of ICAM-1 and ICAM-3 to LFA-1. FACS was performed on 5x105 cultured Molt-4 human tumor cells using anti-CD11a antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
ReferencesHolness, C.L., et al. 1995. J. Biol. Chem. 270, 877.
Landis, R.C., et al. 1994. J. Cell. Biol. 126, 529.
Dransfield, I., and Hogg, N. 1989. EMBO J. 8, 3759.