Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

217669 Anti-CD28 Mouse mAb (ANC28.1/5D10)

Anti-CD28, mouse monoclonal, clone ANC28.1/5D10, recognizes CD28 on human T cells & human CD28 transfected BHK cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Anti-CD28 Mouse mAb (ANC28.1/5D10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
217669
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
217669-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes CD28 on human T cells and BHK cells transfected with human CD28.
Catalogue Number217669
Brand Family Calbiochem®
References
ReferencesKariv, I., et al. 1996. J. Immunol. 157, 29.
61st Forum in Immunology. 1995. Res. Immunol. 146, 127.
Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol. 5, 361.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Preservative500 µg/ml gentamycin sulfate
Quality LevelMQ100
Applications
Key Applications Flow Cytometry
Frozen Sections
Immunoprecipitation
T Cell Co-stimulation
Application NotesFlow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
T cell Co-stimulation (see comments)
Application CommentsMonomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman CD28 Ig fusion protein and HPB-MLT human tumor cells
ImmunogenHuman
CloneANC28.1/5D10
HostMouse
Purity>95% immunoglobulin by SDS-PAGE
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
217669-100UG 04055977202953

Documentation

Anti-CD28 Mouse mAb (ANC28.1/5D10) SDS

Title

Safety Data Sheet (SDS) 

Anti-CD28 Mouse mAb (ANC28.1/5D10) Certificates of Analysis

TitleLot Number
217669

References

Reference overview
Kariv, I., et al. 1996. J. Immunol. 157, 29.
61st Forum in Immunology. 1995. Res. Immunol. 146, 127.
Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol. 5, 361.

Citations

Title
  • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision25-July-2007 RFH
    ApplicationFlow Cytometry (5 µg/ml)
    Frozen Sections (see comments)
    Immunoprecipitation (see comments)
    T cell Co-stimulation (see comments)
    DescriptionProtein A purified mouse monoclonal antibody. Recognizes the CD28 protein.
    BackgroundHuman CD28 is an important co-stimulatory molecule found on all CD4+ T cells and ~50% of CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA, and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, known as CD80 (B7-1) and CD86 (B7-2), are monomeric transmembrane glycoproteins.
    HostMouse
    Immunogen speciesHuman
    Immunogenhuman CD28 Ig fusion protein and HPB-MLT human tumor cells
    CloneANC28.1/5D10
    IsotypeIgG₁
    Specieshuman
    FormLiquid
    FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Purity>95% immunoglobulin by SDS-PAGE
    Preservative500 µg/ml gentamycin sulfate
    CommentsMonomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
    Toxicity Standard Handling
    ReferencesKariv, I., et al. 1996. J. Immunol. 157, 29.
    61st Forum in Immunology. 1995. Res. Immunol. 146, 127.
    Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol. 5, 361.
    Citation
  • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
    		•