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MABF2340

Sigma-Aldrich

Anti-CD300a (CLM-8) Antibody, clone TX41

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

antibody form

purified antibody

clone

TX41
monoclonal

purified by

using protein G

species reactivity

rat, mouse, mouse

concentration

(Please refer to lot specific datasheet.)

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG2aκ

suitability

suitable for immunoassay

UniProt accession no.

General description

Ba/F3 cells transfected with murine CLM-8 (MAIR-I/CD300a) fused with the Fc portion of human IgG.

Specificity

Clone TX41 is a rat monoclonal antibody that specifically detects mouse CD300a (CLM-8).

Application

Flow Cytometry Analysis: A representative lot detected CD300a (CLM-8) in Flow Cytometry applications (Miki, H., et. al. (2015). J Immunol. 194(11):5069-76).

Function Analysis: A representative lot of this antibody increased neutrophil numbers in the peritoneal cavity, improved bacterial clearance, and prolonged survival in mice. (Nakahashi-Oda, C., et. al. (2012). J Exp Med. 209(8):1493-503).


Immunofluorescence Analysis: A representative lot detected CD300a (CLM-8) in Immunofluorescence applications (Miki, H., et. al. (2015). J Immunol. 194(11):5069-76).

Quality

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CD300a (CLM-8) in mouse kidney tissue lysate.

Physical properties

~ 30 kDa observed; 35.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified rat monoclonal antibody in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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