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CBL150-I Anti-CD3e Antibody, clone UCHT1

Anti-CD3e Antibody, clone UCHT1 is an antibody against CD3e for use in Flow Cytometry, Immunoprecipitation, ELISA, Immunocytochemistry, Immunohistochemistry, Affects Function.

Anti-CD3e Antibody, clone UCHT1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: CD3E, T3E UniProt Number: P07766
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CBL150-I
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IP, ELISA, ICC, IHC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL150-I
DescriptionAnti-CD3e Antibody, clone UCHT1
Alternate Names
  • T-cell surface glycoprotein CD3 epsilon chain
  • CD3-epsilon
  • CD3e
  • T-cell surface antigen T3/Leu-4 epsilon chain
Background InformationT-cell surface glycoprotein CD3 epsilon chain (UniProt P07766; also known as CD3-epsilon, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain) is encoded by the CD3E (also known as T3E) gene (Gene ID 916) in human. The T cell receptor or TCR is a plasma membrane protein complex responsible for recognizing antigens bound to major histocompatibility complex (MHC) on antigen presenting cells. In 95% of T cells, the TCR is composed of a disulfide-linked alpha (α) and beta (β) dimer, while among 5% of T cells the TCR is composed of a gamma (γ) and a delta (δ) dimer. T cells are activated when their surface TCRs engage with antigen/MHC complex on antigen presenting cells. Each TCR alpha and beta chain contains a variable (V) and a constant (C) extracellular immunoglobulin-like domain, followed by a transmembrane region and a short cytoplasmic tail. The alpha and beta chains have very short cytoplasmic tail and rely on the CD3 (cluster of differentiation 3) co-receptor complex for transducing signaling further intracellularly. The CD3 co-receptor complex is composed of a CD3γ chain, a CD3δ chain, and two CD3ε chains. The CD3 complex, TCR dimer, and a ζ-chain together comprise the functional TCR complex. The CD3 epsilon chain is initially produced with a signal peptide (a.a. 1-22), the removal of which yields the mature chain (a.a. 23-207) with an Ig-like (a.a. 32-112) domain-containing extracellular region (a.a. 23-126), a transmembrane segment (a.a. 127-152), and a cytoplasmic tail (a.a. 153-207), which contains the immunoreceptor tyrosine-based activation motif or ITAM (a.a. 178-205) that is essential for mediating ZAP-70 interaction and transducing TCR signaling.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-CD3e Antibody, clone UCHT1 is an antibody against CD3e for use in Flow Cytometry, Immunoprecipitation, ELISA, Immunocytochemistry, Immunohistochemistry, Affects Function.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Affects Function
Application NotesImmunoprecipitation Analysis: Representative lots immunoprecipitated CD3 epsilon-associated TCR complex (Schamel, W.W., et al. (2005). J. Exp. Med. 202(4):493-503; Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).
ELISA Analysis: A representative lot was used as the capture antibody for the detection of epsilon-associated TCR complex proteins by sandwich ELISA (Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).
Immunocytochemistry Analysis: A representative lot detected the exogensously expressed human CD3 epsilon chain on the surface of murine T cells by flourescent immunocytochemistry (Clevers, H., et al. (1988). Eur. J. Immunol. 18(5):705-710).
Immunocytochemistry Analysis: A representative lot was employed to determined the plasma membrane and cytoplasmic CD3 immunoreactivity in T cell populations and T-ALL blast cells by flourescent immunocytochemistry (Campana, D., et al. (1987). J. Immunol. 138(2):648-655).
Immunohistochemistry Analysis: A representative lot detected CD3+ T cells (T3+) in the periglandular stromal aggregates in frozen dometrium sections from female donors undergone routine dilation and curettage procedures (Kamat, B.R., and Isaacson, P.G. (1987). Am. J. Pathol. 127(1):66-73).
Affects Function: A representative lot induced surface IL-2R expression and proliferation of human peripheral blood T lymphocytes (Van Wauwe, J.P., et al. (1984). J. Immunol. 133(1):129-132).
Flow Cytometry Analysis: A representative lot was employed for fluorescence activated cell sorting (FACS) of CD3+ human lymphocytes (Beverley, P.C., and Callard, R.E. (1981). Science. 11(4):329-334).
Biological Information
ImmunogenHuman infant thymocytes and lymphocytes from a patient with Sézary disease.
EpitopeExtracellular domain.
CloneUCHT1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone UCHT1 reacts with the extracellular domain of human CD3 epsilon chain (Clevers, H., et al. (1988). Eur. J. Immunol. 18(5):705-710).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD3E
  • T3E
Purification MethodProtein G Purified
UniProt Number
Molecular Weight23.15 kDa (Proform) and 20.74 (mature form) calculated. ~27 kDa reported in UCHT1 immunoprecipitate (Poggi, A., et al. (1996). Int. Immunol. 8(12):1947-1953).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in human PBMC.

Flow Cytometry Analysis: 0.1 µg of this antibody detected CD3e in 1X10E6 human PBMC.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
CBL150-I 04055977329582

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies