Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABF575 Anti-CD4 Antibody (mouse), violetFluor™ 450, clone RM4-5

This rat monoclonal antibody Anti-CD4 (mouse), violetFluor 450, clone RM4-5 validated for use in IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD4.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Cd4 UniProt Number: P06332
View Products on Sigmaaldrich.com
MABF575
25 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIP, IHC, IF, FCRvioletFluor™ 450Monoclonal Antibody
Description
Catalogue NumberMABF575
Trade Name
  • violetFluor
DescriptionAnti-CD4 Antibody (mouse), violetFluor™ 450, clone RM4-5
Alternate Names
  • T-cell surface glycoprotein CD4
  • T-cell differentiation antigen L3T4
  • T-cell surface antigen T4/Leu-3
  • CD4
Background InformationThe RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will “compete” for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
References
Product Information
FormatvioletFluor™ 450
PresentationPurified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal antibody Anti-CD4 (mouse), violetFluor 450, clone RM4-5 validated for use in IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD4.
Key Applications
  • Immunoprecipitation
  • Immunohistochemistry
  • Immunofluorescence
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneRM4-5
Concentration0.2 mg/mL
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Cd4
Purification MethodSize Exclusion Chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD4 in one million C57Bl/6 splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size25 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF575 04053252963346

Related Products & Applications

Related Products

Catalog Number Description
MABF1565 Anti-CD4 (Mouse), APC, clone RM4-5 Antibody
MABF1566 Anti-CD4 (Mouse), APC, clone RM4-5 Antibody
MABF1567 Anti-CD4 (Mouse), FITC, clone RM4-5 Antibody
MABF1568 Anti-CD4 (Mouse), FITC, clone RM4-5 Antibody
MABF1569 Anti-CD4 (Mouse), PE, clone RM4-5 Antibody
MABF1571 Anti-CD4 (Mouse), PE-Cy7, clone RM4-5 Antibody
MABF1573 Anti-CD4 (Mouse), PerCP-Cy5.5, clone RM4-5 Antibody
MABF1574 Anti-CD4 (Mouse), PerCP-Cy5.5, clone RM4-5 Antibody
MABF1576 Anti-CD4 (Mouse), redFluor™ 710, clone GK1.5 Antibody
MABF1577 Anti-CD4 (Mouse), redFluor™ 710 Antibody, clone RM4-5
Show more

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies