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MABF609 Anti-CD45RA Antibody (human), PE-Cy7, clone HI100

This mouse monoclonal antibody Anti-CD45RA (human), PE-Cy7, clone HI100 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD45RA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: PTPRC, CD45 UniProt Number: P08575
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MABF609
25 tests  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IHC, WBMPE-Cy7Monoclonal Antibody
Description
Catalogue NumberMABF609
Replaces04-1102
DescriptionAnti-CD45RA Antibody (human), PE-Cy7, clone HI100
Alternate Names
  • Receptor-type tyrosine-protein phosphatase C
  • Leukocyte common antigen
  • L-CA
  • T200
  • CD antigen CD45
Background InformationThe HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others.The HI100 antibody is widely used as a marker for human CD45RA expression on naïve and activated T cells, B cells, and monocytes.
References
Product Information
FormatPE-Cy7
PresentationPurified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal antibody Anti-CD45RA (human), PE-Cy7, clone HI100 validated for use in western blotting, IHC & Flow Cytometry for the detection of CD45RA.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Western Blotting
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneHI100
Concentration5 µL (0.25 µg)/test
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • PTPRC
  • CD45
Purification MethodSize Exclusion Chromatography
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected CD45RA in one million human peripheral blood lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size25 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF609 04053252965753