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MABT859 Anti-CDCP1 (CD318) Antibody, clone 8A2

Anti-CDCP1 (CD318), clone 8A2, Cat. No. MABT859, is a mouse monoclonal antibody that detects CUB domain-containing protein 1 (CDCP1) and has been tested for use Flow Cytometry.

Anti-CDCP1 (CD318) Antibody, clone 8A2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: CDCP1, TRASK UniProt Number: Q9H5V8
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MABT859
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT859
DescriptionAnti-CDCP1 (CD318) Antibody, clone 8A2
Alternate Names
  • CUB domain-containing protein 1
  • Membrane glycoprotein gp140
  • Subtractive immunization M plus HEp3-associated 135 kDa protein
  • SIMA135
  • Transmembrane and associated with src kinases
  • CD318
Background InformationCUB domain-containing protein 1 (UniProt: Q9H5V8; also known as Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with Src kinases, CD318) is encoded by the CDCP1 (also known as TRASK) gene (Gene ID: 64866) in human. CUB domain-containing protein 1 is a single-pass membrane protein that is synthesized with a 29 amino acid signal peptide, which is subsequently cleaved to generate the mature form. A soluble form of CUB domain-containing protein 1 has also been reported that is produced by proteolytic cleavage at the cell surface. CUB domain-containing protein 1 is highly expressed in mitotic cells with low expression during interphase. It is detected at highest levels in skeletal muscle and colon. Lower expression is reported in kidney, small intestine, placenta, and lung. It is also reported in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. CUB domain-containing protein 1 levels are up-regulated in a number of human tumor cell lines and in colorectal cancer, breast carcinoma and lung cancer. It is reported to be involved in cell adhesion and cell matrix association and may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CDCP1 (CD318), clone 8A2, Cat. No. MABT859, is a mouse monoclonal antibody that detects CUB domain-containing protein 1 (CDCP1) and has been tested for use Flow Cytometry.
Key Applications
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A representative lot detected CDCP1 (CD318) in one million MDA and MDA KO cells (Courtesy of the Laboratory of Feng Lin, Ph.D., Cleveland Clinic, Lerner Research Institute, Cleveland OH USA).
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 638 amino acids from the extracellular domain of human CUB domain-containing protein 1.
Epitopeextracellular domain
Clone8A2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 8A2 detects CUB domain-containing protein 1 (CDCP1) in human cells. it targets an epitope within the extracellular domain.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CDCP1
  • TRASK
Purification MethodProtein G purified
UniProt Number
Molecular Weight92.93 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in DLD1 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected CDCP1 (CD318) in one million DLD1 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT859 04054839355608