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MABT1525-100UG Anti-CDH17 Antibody, clone Lic5

Anti-CDH17, clone Lic5, Cat. No. MABT1525, is a mouse monoclonal antibody that detects Cadherin-17 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Inhibition Assays, and Western Blotting.

MABT1525-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1525-100UG
DescriptionAnti-CDH17 Antibody, clone Lic5
Alternate Names
  • Intestinal peptide-associated transporter HPT-1
  • Liver-intestine cadherin
  • LI-cadherin
Background InformationCadherin-17 (UniProt: Q12864; also known as Intestinal peptide-associated transporter HPT-1, Liver-intestine cadherin, LI-cadherin) is encoded by the CDH17 gene (Gene ID: 1015) in human. Cadherin-17 is a single-pass type I membrane protein that is expressed in the gastrointestinal tract and pancreatic duct. It belongs to the non-classical cadherin with distinct structural feature of having seven cadherin repeats at its extracellular amino-terminus. Three calcium ions are usually bound at the interface of each cadherin repeat and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Cadherin-17 is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature form that contains a long extracellular domain (aa 23-787), a transmembrane domain (aa 788-808), and a cytoplasmic domain (aa 809-832). It differs from classical cadherins in having a short cytoplasmic tail at its C-terminus. Cadherin-17 is reported to be a oncofetal protein that is associated with poor prognostic outcome in subjects with hepatocellular carcinoma. Its levels can be up-regulated or down-regulated in different cancers, including pancreatic, gastric and colorectal cancers. Clone Lic5 is shown to reduce the levels of beta-catenin and in hepatocellular carcinoma cells and administration of Lic5 is reported to induce the activation of caspase-8 and 9 in these cells. In addition, it is also shown to suppress tumor growth of MHCC97L cells in murine models. (Ref.: Wang, Y., et al. (2013). PLoS One. 8(8); e72386).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2bk in buffer containing PBS without azide.
Applications
ApplicationAnti-CDH17, clone Lic5, Cat. No. MABT1525, is a mouse monoclonal antibody that detects Cadherin-17 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Inhibition Assays, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Inhibition
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected CDH17 in Immunohistochemistry applications (Wang, Y., et. al. (2013). PLoS One. 8(9):e72386).

Western Blotting Analysis: A representative lot detected CDH17 in Western Blotting applications (Wang, Y., et. al. (2013). PLoS One. 8(9):e72386).

Immunocytochemistry Analysis: A representative lot detected CDH17 in Immunocytochemistry applications (Wang, Y., et. al. (2013). PLoS One. 8(9):e72386).

Inhibition Analysis: A representative lot reduced CDH17 expression in a dose-dependent manner and suppressed -catenin signaling. (Wang, Y., et. al. (2013). PLoS One. 8(9):e72386).
Biological Information
ImmunogenRecombinant fragment corresponding to extracellular domains 1 and 2 of human Cadherin-17 (CDH17).
Epitopeextracellular domain
CloneLic5
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone Lic5 is a mouse monoclonal antibody that specifically detects human Cadherin-17. It targets an epitope within the extracellular domain.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CDH17
Purification MethodProtein G purified
UniProt Number
Molecular Weight92.22 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human small intestine tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Cadherin-17 (CDH17) in glandular cells of human small intestine tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT1525-100UG 04061841068655

Documentation

Anti-CDH17 Antibody, clone Lic5 SDS

Title

Safety Data Sheet (SDS) 

Anti-CDH17 Antibody, clone Lic5 Certificates of Analysis

TitleLot Number
Anti-CDH17, clone Lic5 - Q3286539 Q3286539

References

Reference overviewPub Med ID
Anti-cadherin-17 antibody modulates beta-catenin signaling and tumorigenicity of hepatocellular carcinoma.
Wang, Y; Shek, FH; Wong, KF; Liu, LX; Zhang, XQ; Yuan, Y; Khin, E; Hu, MY; Wang, JH; Poon, RT; Hong, W; Lee, NP; Luk, JM
PLoS One  8  e72386  2013

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