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MABF917 Anti-CRACC Antibody, clone 4G2

Anti-CRACC Antibody, clone 4G2 is an antibody against CRACC for use in Flow Cytometry, Affects Function, Immunoprecipitation.

Anti-CRACC Antibody, clone 4G2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: Slamf7, Cs1 UniProt Number: Q8BHK6
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MABF917
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, FUNC, IPRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF917
DescriptionAnti-CRACC Antibody, clone 4G2
Alternate Names
  • SLAM family member 7
  • CD2-like receptor-activating cytotoxic cells
  • CRACC
  • Leukocyte cell-surface antigen
  • Novel Ly9
  • CD319
Background InformationSLAM family member 7 (UniProt Q8BHK6; also known as CD2-like receptor-activating cytotoxic cells, CRACC, Leukocyte cell-surface antigen, Novel Ly9, CD319) is encoded by the Slamf7 (also known as Cs1) gene (Gene ID 75345) in murine species. CRACC/CS1 belongs to the 6-member (SLAM/CD150, Ly-9/CD229, 2B4/CD244, CD84, NTB-A/CD352, CRACC) signaling lymphocytic activation molecule family (SLAMF) of receptors broadly expressed in hematopoietic cells. 2B4 and CD48 interact with each other, while rest of the family members act as self-ligands, where two identical molecules on the surface of two different cells can interact as receptor and ligand to each other. CRACC is synthesized with a signal peptide sequence (a.a. 1-22), the removal of which yields the mature CRACC with an extracellular region (a.a. 23-224) that contains a V-type and a C2-type Ig-like domain (a.a. 27-112 and 128-203), followed by a single transmembrane domain (a.a. 225-245) and a cytoplasmic tail (a.a. 246-333). CRACC cytoplasmic domain contains one immunoreceptor tyrosine-based switch motif (ITSM; 278-TFYSTV-283, numbering based on mature murine sequence), which upon phosphorylation (pY300) recruits SAP family adaptor EWS-Fli1-activated transcript-2 (EAT-2) for signaling transduction. CRACC can also be phosphorylated on Tyr280. Unlike pY300, pY280 mediates the inhibitory function of CRACC in NK cells. In addition to NK cells, evidence indicates that CRACC is also involved in regulating B- and T-cell functions.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG1κ antibody in PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-CRACC Antibody, clone 4G2 is an antibody against CRACC for use in Flow Cytometry, Affects Function, Immunoprecipitation.
Key Applications
  • Flow Cytometry
  • Affects Function
  • Immunoprecipitation
Application NotesFlow Cytometry Analysis: A representative lot immunostained murine T lymphoma BI-141 transfectants expressing mouse CRACC but not those transfected with other members of the SLAM family (CD84, 2B4, Ly9, Ly108 or SLAM) (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
Flow Cytometry Analysis: A representative lot detected CRACC expression on the surface of many types of mouse immune system cells, including NK cells, NKT cells, B cells, DCs, macrophages and activated T cells (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
Affects Function: A representative lot, in combination with a rabbit anti-rat IgG, cross-linked cell surface CRACC and induced similar CRACC phosphorylation in IL-2-activated NK cells from wild-type, SAP-deficient, or EAT-2–ERT–double knock (DKO) mice (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
Immunoprecipitation Analysis: A representative lot, in combination with a rabbit anti-rat IgG and protein A, immunoprecipitated CRACC from lysates of IL-2-activated mouse NK cells (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305).
Biological Information
ImmunogenHuman IgG1 Fc -tagged recombinant mouse CRACC extracellular domain.
EpitopeExtracellular domain.
Clone4G2
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 4G2 reacted with murine T lymphoma BI-141 transfectants expressing mouse CRACC but not with those transfected with other members of the SLAM family (CD84, 2B4, Ly9, Ly108 or SLAM) (Cruz-Munoz, M.E., et al. (2009). Nat. Immunol. 10(3):297-305). Expected to react with all four spliced isoforms of murine CRACC reported by UniProt (Q8BHK6).
IsotypeIgG1κ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Slamf7
  • Cs1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight34.77 kDa (isoform 1), 34.87 kDa (isoform 2), 30.71 kDa (isoform 3), and 30.61 kDa (isoform 4) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 0.1 µg of this antibody detected CRACC-positive mouse splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF917 04055977349627