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ABE553 Anti-CREB-1 Antibody

Detect CREB using this rabbit polyclonal antibody, Anti-CREB-1 Antibody validated for use in western blotting & ICC.

Anti-CREB-1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: CREB1 UniProt Number: P16220
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ABE553
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, ICC, ChIPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE553
Replaces04-218
DescriptionAnti-CREB-1 Antibody
Alternate Names
  • Cyclic AMP-responsive element-binding protein 1
  • CREB-1
  • cAMP-responsive element-binding protein 1
Background InformationCyclic AMP-responsive element-binding protein (CREB) is a beta ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2.
References
Product Information
FormatAffinity Purified
Control
  • A431 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect CREB using this rabbit polyclonal antibody, Anti-CREB-1 Antibody validated for use in western blotting & ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Chromatin Immunoprecipitation (ChIP)
Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CREB-1 in PC-12 cells.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CREB-1 in HEK293T cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human CREB-1.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the N-terminus of CREB-1.
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteDemonstrated to react with Human and Rat. Predicted to react with Porcine and Equine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CREB1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~51 kDa observed. A doublet has been observed at 48 kDa in some cell lysates (Rutberg, S. E., et al. (1999). Oncogene. 18(8):1569-1579.). Uncharacterized bands may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CREB-1 in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE553 04053252888564

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies