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MABS1163 Anti-CSF-1R/CD115 Antibody clone FER216

Anti-CSF-1R/CD115 Antibody clone FER216 is an antibody against CSF-1R for use in Western Blotting.

Anti-CSF-1R/CD115 Antibody clone FER216 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytokines & Cytokine Receptors Gene Symbol: CSF1R, CSFR, FMS, HDLS UniProt Number: P07333
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MABS1163
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1163
DescriptionAnti-CSF-1R/CD115 Antibody clone FER216
Alternate Names
  • Macrophage colony-stimulating factor 1 receptor
  • CD115
  • CSF-1 receptor
  • CSF-1-R
  • CSF-1R
  • M-CSF-R
  • Proto-oncogene c-Fms
Background InformationMacrophage colony-stimulating factor 1 receptor (EC 2.7.10.1; UniProt P07333; also known as CD115, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms) is encoded by the CSF1R (also known as CSFR, FMS, HDLS) gene (Gene ID 1436) in human. CSF1R is a CSF1/PDGF family receptor tyrosine kinase that mediates macrophage colony stimulating factor 1 (M-CSF1) and IL-34 signaling by acting as the receptor for these two cytokines. CSF1R plays a key role in the differentiation, recruitment and activation of tissue macrophages. Tumor-associated macrophages (TAMs) have been associated with survival in classic Hodgkin lymphoma (cHL) and other lymphoma types. Hyperphosphorylated CSF1R is found in cHL tissue and highlights the importance of CSF1/CSF1R signaling in the recruitment of TAMs. CSF1R is initially produced with a signal peptide sequence (a.a. 1-19), the removal of which yields the mature type I single transmembrane protein with an extracellular region (a.a. 20-517), a transmembrane segment (a.a. a.a. 518-538), and a cytoplasmic region (a.a. 539-972) that contains the kinase domain (a.a. 582-910).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CSF-1R/CD115 Antibody clone FER216 is an antibody against CSF-1R for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected CSF-1R/CD115 in 10 µg of human tonsil tissue lysate.
Biological Information
ImmunogenHuman IgG1 Fc-tagged recombinant human CSF-1R/CD115 extracellular domain.
EpitopeExtracellular domain.
CloneFER216
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone FER216 reacts with the extracellular domain of human CSFR1, but not the extracellular domain of human FGFR1. Clone FER216 detected the ~100 kDa human CSFR1 in nomral tonsil and Hodgkin lymphoma (cHL) tissue extracts, as well as an additional ~150 kDa phosphorylated CSFR1 band in cHL tissue extracts (Martín-Moreno, A.M., et al. (2015). PLoS One. 2015 Jun 12;10(6):e0125203).
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CSF1R
  • CSFR
  • FMS
  • HDLS
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~107 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human colon tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CSF-1R/CD115 in 10 µg of human colon tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS1163 04055977314281

Documentation

Anti-CSF-1R/CD115 Antibody clone FER216 SDS

Title

Safety Data Sheet (SDS) 

Anti-CSF-1R/CD115 Antibody clone FER216 Certificates of Analysis

TitleLot Number
Anti-CSF-1R/CD115, clone FER216 - 3174760 3174760
Anti-CSF-1R/CD115, clone FER216 - 3280061 3280061
Anti-CSF-1R/CD115, clone FER216 - 3862594 3862594
Anti-CSF-1R/CD115, clone FER216 - 3906402 3906402
Anti-CSF-1R/CD115, clone FER216 - 3991572 3991572
Anti-CSF-1R/CD115, clone FER216 - 4023281 4023281
Anti-CSF-1R/CD115, clone FER216 - 4061835 4061835
Anti-CSF-1R/CD115, clone FER216 -2751108 2751108
Anti-CSF-1R/CD115, clone FER216 -2762050 2762050
Anti-CSF-1R/CD115, clone FER216 -Q2641929 Q2641929
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