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ABN182 Anti-CYP27B1 Antibody

This Anti-CYP27B1 Antibody is validated for use in Western Blotting, IHC for the detection of CYP27B1.

Anti-CYP27B1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: CYP27B1, CYP1ALPHA, CYP27B UniProt Number: O15528
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ABN182
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN182
DescriptionAnti-CYP27B1 Antibody
Alternate Names
  • 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
  • 25-OHD-1 alpha-hydroxylase
  • 25-hydroxyvitamin D(3) 1-alpha-hydroxylase
  • VD3 1A hydroxylase
  • Calcidiol 1-monooxygenase
  • Cytochrome P450 subfamily XXVIIB polypeptide 1
  • Cytochrome P450C1 alpha
  • Cytochrome P450VD1-alpha
  • Cytochrome p450 27B1
Background Information25-hydroxyvitamin D-1 alpha hydroxylase (1αOHase; VD3 1A hydroxylase; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; or CYP27B1) is a mitochondrial enzyme that belongs to the cytochrome P450 family. It catalyzes the hydroxylation of 25-hydroxyvitamin D3 to form an active 1-alpha, 25-dihydroxyvitamin D3, in the kidneys and other tissues. 25-hydroxyvitamin D-1 alpha hydroxylase plays an important role in bone growth, calcium metabolism, and tissue differentiation. Previous studies have reported that mice deficient in 25-hydroxyvitamin D-1 alpha hydroxylase are affected by hypocalcemia, secondary hyperparathyroidism, retarded growth, and the skeletal abnormalities. In humans, defects in this enzyme causes pseudovitamin D deficiency rickets (vitamin D-dependent type 1A rickets), an autosomal recessive condition characterized by abnormal bone mineralization and impaired growth.
References
Product Information
FormatAffinity Purified
Control
  • Serum free MCF-7 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-CYP27B1 Antibody is validated for use in Western Blotting, IHC for the detection of CYP27B1.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CYP27B1 in mouse and rat hippocampus tissue and in rat kidney tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human CYP27B1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat. Predicted to react with Chimpanzee based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CYP27B1
  • CYP1ALPHA
  • CYP27B
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~58 kDa observed. Uncharacterized bands may be observed at ~55 kDa, ~70 kDa, ~110 kDa, and ~115 kDa in some cell lysates. A doublet at ~58 kDa may be observed in some cell lysates (Kemmis, C. M., et al. (2006). J Nutr. 136(4):887-892.).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in serum free MCF-7 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected CYP27B1 in 10 µg of serum free MCF-7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABN182 04053252696770