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MAC133 Anti-Cas9 Antibody, clone 7A9

Anti-Cas9 Antibody, clone 7A9 | MAC133 is an antibody against Cas9 Antibody for use in Western Blotting, Immunoprecipitation and Immunocytochemistry.

Anti-Cas9 Antibody, clone 7A9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Research Sub-Category: Secondary Antibodies Adsorbed for Dual Labeling
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MAC133
100 µg  
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Overview

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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, IP, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAC133
DescriptionAnti-Cas9 Antibody, clone 7A9
Alternate Names
  • dCas9
Background InformationCas9 is an essential endonuclease in Streptococcus pyogenes serotype M1 that is part of that organism’s innate immunity system, or CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or to help distinguish self versus nonself. Researchers hope to exploit the programmability and precise nature of Cas9 cleavage for more effective recombinant DNA technologies.
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Cas9 Antibody, clone 7A9 | MAC133 is an antibody against Cas9 Antibody for use in Western Blotting, Immunoprecipitation and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Cas9 in 1 µg of Myc-Cas9 expressing HEK293 cell lysate.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).
Biological Information
ImmunogenN-Terminal domain of Cas9.
Clone7A9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityMAC133 recognises both Cas9 and dCas9 (nuclease deficient Cas9)
IsotypeIgG1κ
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular Weight~160 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 expressing Flag-Cas9 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cas9 in 1 µg of HEK293 cell lysate expressing Flag-Cas9.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAC133 04055977127034

Documentation

Anti-Cas9 Antibody, clone 7A9 SDS

Title

Safety Data Sheet (SDS) 

Anti-Cas9 Antibody, clone 7A9 Certificates of Analysis

TitleLot Number
Anti-Cas9, clone 7A9 3167596
Anti-Cas9, clone 7A9 - 2532575 2532575
Anti-Cas9, clone 7A9 - 3205439 3205439
Anti-Cas9, clone 7A9 - 3256668 3256668
Anti-Cas9, clone 7A9 - 3313137 3313137
Anti-Cas9, clone 7A9 - 3482825 3482825
Anti-Cas9, clone 7A9 - 3681278 3681278
Anti-Cas9, clone 7A9 - 3835371 3835371
Anti-Cas9, clone 7A9 - 3874277 3874277
Anti-Cas9, clone 7A9 - 3915598 3915598
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