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MAC148-25UG Anti-Cas9 Antibody, clone 8G4

Anti-Cas9, clone 8G4, Cat. No. MAC148, is a rat monoclonal antibody that detects Cas9 and is tested for use in Western Blotting and Immunofluorescence.

NewMAC148-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAC148-25UG
DescriptionAnti-Cas9 Antibody, clone 8G4
Alternate Names
  • CRISPR-associated endonuclease Cas9/Csn1
  • SpCas9
  • SpyCas9
Background InformationCRISPR-associated endonuclease Cas9/Csn1 (UniProt: Q99ZW2; also known as SpCas9, SpyCas9, Cas9) is encoded by the Cas9 gene (Gene ID: 69900935) in Streptococcus pyogenes. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements, and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bi-lobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. (Ref.: Fonfara, I., et al. (2014). Nucleic Acids Res. 42(4); 2577-2590; Jinek, M., et al. (2012). Science. 337(6096); 816-821).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Cas9, clone 8G4, Cat. No. MAC148, is a rat monoclonal antibody that detects Cas9 and is tested for use in Western Blotting and Immunofluorescence.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesTested Applications

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Cas9 in Cell line stably expressing Cas9-NLS (A) versus wild type parental cell line (B) (Courtesy of Germán Camargo Ortega (Institute of Stem Cell Research, Helmholtz Munich,. Germany).

Western Blotting Analysis: A representative lot detected Cas9 in Western Blotting application. (Camargo Ortega, G., et al. (2019). Nature. 567(7746):113-117).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenHis-tagged, full-length, recombinant Cas9 from Streptococcus pyogenes.
EpitopeUnknown
Clone8G4
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 8G4 is a rat monoclonal antibody that specifically detects Cas9.
IsotypeIgG2aλ
Species Reactivity
  • All
Species Reactivity NoteAll.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Cas9
Purification MethodProtein G purified
UniProt Number
Molecular Weight~200 kDa observed; 158.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HeLa cells overexpressing Cas9.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cas9 in lysate from HeLa cells overexpressing Cas9.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAC148-25UG 04065271175112