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ABC495 Anti-Caspase 3 Antibody

Anti-Caspase 3 Antibody is an antibody against Caspase 3 Antibody for use in Western Blotting and Immunocytochemistry.

Anti-Caspase 3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: CASP3, CPP32 UniProt Number: P42574
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ABC495
50 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC495
Replaces06-375
DescriptionAnti-Caspase 3 Antibody
Alternate Names
  • Caspase-3
  • CASP-3
  • Apopain
  • Cysteine protease CPP32
  • CPP-32
  • Protein Yama
  • SREBP cleavage activity 1
  • SCA-1
  • Caspase-3 subunit p17
  • Caspase-3 subunit p12
  • Caspase 3
Background InformationCaspase-3 (EC 3.4.22.56; UniProt P42574; also known as Apopain, CASP-3, CPP-32, PARP cleavage protease, SCA-1, SREBP cleavage activity 1, Caspase 3 apoptosis-related cysteine protease, Cysteine protease CPP32, Procaspase3, Protein Yama) is encoded by the CASP3 (also known as CPP32, CPP32B) gene (Gene ID 836) in human. Cysteine-aspartic proteases or Caspases play essential roles in apoptosis, necrosis, and inflammation. Historically, caspases were numbered in the order in which they were identified. Caspase-3 is initially produced with a propeptide sequence (a.a. 1-9), the removal of which yields the 268 a.a. caspase-3 proenzyme. Upon activation, the proenzyme is proteolytically cleaved first between Asp175-Ser176 to generate a p20 (a.a. 10-175) fragment and the p12 (a.a. 176-277) subunit. Further cleavage of the p20 fragment between Asp28-Ser29 produces the p17 (a.a. 29-175) subunit. The p17 and p12 dimerizes and forms the active caspase-3 enzyme. Caspase-3 plays a central role in the sequential activation of caspases during the execution phase of cell apoptosis. Caspase-3 mediates the proteolytic activation of caspases-6 and -7, while caspase-3 itself is processed and activated by caspase-8, -9, or -10. Caspase-3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Caspase 3 Antibody is an antibody against Caspase 3 Antibody for use in Western Blotting and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected Caspase 3 in HeLa, A431, HUVEC, and NIH/3T3 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Caspase 3 near the C-terminus.
EpitopeNear C-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with pro-caspase 3, the uncleaved/inactive caspase 3 (32 kDa), and the p12 (12 kDa), but not the p17 (17 kDa) subunit of cleaved/active caspase 3.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Non-Human Primate, Canine, Feline, Hamster based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CASP3
  • CPP32
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~32 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected Caspase 3 in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABC495 04055977294811