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PC41 Anti-Cathepsin B (Ab-3) Rabbit pAb

Anti-Cathepsin B (Ab-3), rabbit polyclonal, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in breast carcinoma. It is validated for Western blotting and for paraffin sections.

Anti-Cathepsin B (Ab-3) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC41
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
PC41-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in breast carcinoma tissues.
Catalogue NumberPC41
Brand Family Calbiochem®
References
ReferencesBarrett, A.J. and Kirschke, H. 1981. Methods Enzymol. 80, 535.
Chan, S.J., et al. Proc. Natl. Acad. Sci. USA 83, 7721.
Barrett, A.J. 1987. Trends Biochem. Sci. 12, 193.
Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 137.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive controlSK-HEP-1 concentrated conditioned medium or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1 µg/ml)
Paraffin Sections (2.5 µg/ml, trypsin pre-treatment required)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified human liver cathepsin B
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PC41-100UG 04055977224597

Documentation

Anti-Cathepsin B (Ab-3) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Cathepsin B (Ab-3) Rabbit pAb Certificates of Analysis

TitleLot Number
PC41

References

Reference overview
Barrett, A.J. and Kirschke, H. 1981. Methods Enzymol. 80, 535.
Chan, S.J., et al. Proc. Natl. Acad. Sci. USA 83, 7721.
Barrett, A.J. 1987. Trends Biochem. Sci. 12, 193.
Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 137.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-October-2007 RFH
ApplicationImmunoblotting (1 µg/ml)
Paraffin Sections (2.5 µg/ml, trypsin pre-treatment required)
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.
BackgroundCathepsin B (CB) is a normal lysosomal proteinase which is expressed in all cells. It is a cysteine endoproteinase which is structurally and functionally related to the papain family of proteinases. CB is synthesized as an inactive ~43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of ~31 kDa or the two-chain form (~25 and ~5 kDa subunits). Mature CB is normally localized in the lysosomes where it functions in protein turnover. It has also been shown to be capable of degrading extracellular matrix proteins at acidic and neutral pH. Endogenous inhibitors of cysteine proteinases, the cystatins and stefins, may play a major role in regulating their activity. In tumor cells and in cells exposed to mitogens, CB displays altered cellular trafficking resulting in the secretion of the ~43 kDa precursor. Elevated levels of CB correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.
HostRabbit
Immunogen speciesHuman
Immunogenpurified human liver cathepsin B
IsotypeIgG
Specieshuman
Positive controlSK-HEP-1 concentrated conditioned medium or breast carcinoma tissue
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesBarrett, A.J. and Kirschke, H. 1981. Methods Enzymol. 80, 535.
Chan, S.J., et al. Proc. Natl. Acad. Sci. USA 83, 7721.
Barrett, A.J. 1987. Trends Biochem. Sci. 12, 193.
Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 137.