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MABS2153-25UG Anti-Cep55 Antibody, clone 5A3

Anti-Cep55, clone 5A3, Cat. No. MABS2153, is a mouse monoclonal antibody that detects Centrosomal protein of 55 kDa (Cep55) and has been tested for use in Western Blotting.

MABS2153-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2153-25UG
DescriptionAnti-Cep55 Antibody, clone 5A3
Alternate Names
  • Centrosomal protein of 55 kDa
  • Up-regulated in colon cancer 6
  • FLJ10540
Background InformationCentrosomal protein of 55 kDa (UniProt: Q53EZ4; also known as Cep55, Upregulated in colon cancer 6) is encoded by the CEP55 (also known as C10orf3, URCC6) gene (Gene ID: 55165) in human. Cep55 is a homodimeric protein that plays a role in mitotic exit and successful completion of cytokinesis. It is located in the centrosome in interphase cells and recruited to the midbody during cytokinesis. Cep55 is highly expressed in testis and its expression is also reported in fetal brain ganglionic eminence, kidney tubules, and multinucleate neurons in the temporal cortex. Its expression has also been reported in several cancer cell lines. Cep55 is present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas most of it localizes to the pericentriolar material. During mitosis, it loses its affinity for the centrosome at the onset of prophase and diffuses throughout the cell. This dissociation from the centrosome is phosphorylation dependent. In Cep55, serine 425 and 428 are shown to be phosphorylated at the onset of mitosis and prior to serine 436 phosphorylation. These phosphorylations are essential for dissociation from the centrosome at the G2/M boundary. In Western blot analysis of synchronized cells, Cep55 may appear as a doublet that is indicative of phosphorylation during mitosis, and a single protein in interphase cells. In cancer cells, Cep55 expression is correlated with cell growth rate and p53 is shown to negatively regulate its levels and promoter activity. Down-regulation of Cep55 is accompanied by repression of polo-like kinase 1 (PLK1). Over-expression of PLK1 and knockdown of p53 are reported to enhance the post-translational stability of Cep55. (Ref.: Chang, YC., et al. (2010). J. Cell. Biochem. 109(6); 1274 1285; Chang, YC., et al. (2012). J. Biol. Chem. 287(6); 4376-4385).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Cep55, clone 5A3, Cat. No. MABS2153, is a mouse monoclonal antibody that detects Centrosomal protein of 55 kDa (Cep55) and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected Cep55 in HeLa cell lysate.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length recombinant human Centrosomal protein of 55 kDa (Cep55).
EpitopeUnknown
Clone5A3
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5A3 is a mouse monoclonal antibody that detects Centrosomal protein of 55 kDa (Cep55).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CEP55
  • C10orf3
  • URCC6
Purification MethodProtein G purified
UniProt Number
Molecular Weight~55 kDa observed; 54.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected Cep55 in Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS2153-25UG 04061841879688