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ABF1168-100UL Anti-Chitinase-1 (CHIT1)

Anti-Chitinase-1 (CHIT1), Cat. No. ABF1168, is a rabbit polyclonal that detects Chitinase-1 in human cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Chitinase-1 (CHIT1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CHIT1 UniProt Number: Q13231
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ABF1168-100UL
100 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABF1168-100UL
DescriptionAnti-Chitinase-1 (CHIT1)
Alternate Names
  • Chitotriosidase-1
  • EC: 3.2.1.14
  • Chitinase-1
Background InformationChitotriosidase-1 (UniProt: Q13231; also known as EC: 3.2.1.14, Chitinase-1) is encoded by the CHIT1 gene (Gene ID: 1118) in human. Chitinase-1 is a member of the Chitinase class II subfamily that degrades chitin, chitotriose and chitobiose. It is one of the most abundantly secreted protein and is mainly produced by activated macrophages and epithelial cells. Chitinase -1 can hydrolyze the beta-(1, 4)-linkage between the adjacent N-acetyl glucosamine residues of chitin. It may also participate in the defense against nematodes and other pathogens. Four isoforms of Chitinase-1 have been described that are produced by alternative splicing and isoform 3, which lacks amino acids 344-372, does not possess any enzymatic activity. Chitinase-1 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved to generate the mature form. Its chitin-binding domain is located in amino acids 417-466 and has three chitooligosaccharide binding regions (aa 7-71; 97-100; and 210-213). Elevated levels of Chitinase-1 are observed in cases of Gaucher's disease, bronchial asthma, and atherosclerosis. (Ref.: Kanneganti, M., et al. (2012). J. Epithel. Biol. Pharmacol. 5; 1-9).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Chitinase-1 (CHIT1), Cat. No. ABF1168, is a rabbit polyclonal that detects Chitinase-1 in human cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Chitinase-1 (CHIT1) in human spleen and bone marrow tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Chitinase-1.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Chitinase-1 in human serum. It targets an epitope within 15 amino acids from the C-terminal region.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CHIT1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~ 52 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human serum.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Chitinase-1 in human serum.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABF1168-100UL 04054839448119