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MAB2259 Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7

Detect Cholesterol-24 Hydroxylase using this Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 validated for use in WB, IP, IC, IH.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: CH24H, CP46, CYP46, CYP46A1 UniProt Number: Q9Y6A2
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MAB2259
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, R, RbWB, IP, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2259
DescriptionAnti-Cholesterol-24 Hydroxylase Antibody, clone1A7
Alternate Names
  • Cytochrome P450 46A1
  • cholesterol 24-hydroxylase
  • cytochrome P450, family 46
  • cytochrome P450, family 46, subfamily A, polypeptide 1
  • cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
Background InformationIn enzymology, a cholesterol 24-hydroxylase (EC 1.14.13.98) is an enzyme that catalyzes the chemical reaction:
cholesterol + NADPH + H+ + O2 --> (24S)-24-hydroxycholesterol + NADP+ + H2O. Cholesterol 24-hydroxylase, a cytochrome P450 (CYP46A1), is expressed at 100-fold higher levels in the brain than in the liver. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. Studies indicate that cholesterol 24-hydroxylase constitutes a major tissue-specific pathway for cholesterol turnover in the brain (Lund, 2003).
References
Product Information
FormatPurified
Control
  • Mouse brain lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Cholesterol-24 Hydroxylase using this Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 validated for use in WB, IP, IC, IH.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
  • Immunohistochemistry
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to human cholesterol-24 hydroxylase.
Epitopeunknown
Clone1A7
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes cholesterol-24 hydroxylase.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
  • Rat
  • Rabbit
Species Reactivity NoteDemonstrated to react with mouse. Predicted to react with human, rat, and rabbit based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq].
Gene Symbol
  • CH24H
  • CP46
  • CYP46
  • CYP46A1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol.

CATALYTIC ACTIVITY: Cholesterol + NADPH + O2 = (24S)-24-hydroxycholesterol + NADP+ + H2O.

COFACTOR: Heme group By similarity.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.

TISSUE SPECIFICTY: Expressed in brain. The mRNA was broadly distributed with higher levels in gray matter zones and lower levels in regions rich in white matter. Not detected in fetal sample but its expression increases linearly with age.

SEQUENCE SIMILARITIES: Belongs to the cytochrome P450 family.

BIOPHYSICOCHEMICAL PROPERTIEIS: Kinetic parameters:

KM=2.2 µM for 24(S)-hydroxycholesterol
Molecular Weight~ 54 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Cholesterol-24 hydroxylase mouse brain lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected Cholesterol-24 hydroxylase on 10 µg of Cholesterol-24 hydroxylase mouse brain lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB2259 04053252467332

Documentation

Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 SDS

Title

Safety Data Sheet (SDS) 

Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 Certificates of Analysis

TitleLot Number
Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 2443060 2443060
Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 2006954 2006954
Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 2021742 2021742
Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 2197287 2197287
Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3185040 3185040
Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3189711 3189711
Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3281613 3281613
Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3682587 3682587
Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3719621 3719621
Anti-Cholesterol-24 Hydroxylase, clone 1A7 - 3789406 3789406
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