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AB758 Anti-Collagen Type I Antibody

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AB758
200 µg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, HDB, ELISA, ICC, IHC, WBGtAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB758
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Collagen Type I Antibody
References
Product Information
FormatAffinity Purified
PresentationAffinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen types II, III, IV, V and VI immobilized on Sepharose™ 4B.. Liquid in 0.5 mL of 100 mM borate buffered saline, pH 8.0, no preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-Collagen Type I Antibody is an antibody against Collagen Type I for use in DB, ELISA, IC, IH & WB.
Key Applications
  • Dot Blot
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Applications Not Recommended
  • Immunohistochemistry (Paraffin)
Application NotesDot and slot blotting: 1:100-1:500

ELISA: 1:50-1:100

Indirect immunohistochemistry (frozen sections only): 1:10-1:20

Indirect immunocytochemistry: 1:10-1:20

Antibody reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts {LMF, personal communication}.

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHuman placental collagen type I isolated by limited pepsin digestion and selective salt precipitation.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostGoat
SpecificityRecognizes Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
Species Reactivity
  • Bovine
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]
Gene Symbol
  • COL1A1
  • OI4
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P02452 # Type I collagen is a member of group I collagen (fibrillar forming collagen).
SIZE: 1464 amino acids; 138911 Da
SUBUNIT: Trimers of one alpha 2(I) and two alpha 1(I) chains. Interacts with MRC2 (By similarity).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
TISSUE SPECIFICITY: Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
PTM: Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
DISEASE: SwissProt: P02452 # Defects in COL1A1 are the cause of Caffey disease [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age. & Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type I (EDS-I) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. Ehlers-Danlos syndrome is a genetically and phenotypically heterogeneous connective-tissue disorder characterized by loose- jointedness and fragile, velvety, stretchable, bruisable skin that heals with peculiar 'cigarette-paper' scars. EDS-I is an autosomal dominant trait. & Defects in COL1A1 are a cause of autosomal dominant Ehlers-Danlos syndrome type VII (EDS-VII) [MIM:130060]; which includes also Ehlers-Danlos syndrome type VII-A1. EDS-VII is characterized by arthrochalasis multiplex congenita, skin hyperextensibility and bruisability. & Defects in COL1A1 are a cause of osteogenesis imperfecta type I (OI-I) [MIM:166200]. OI-I is a dominantly inherited serious newborn disease characterized by bone fragility, normal stature, little or no deformity, blue sclerae and hearing loss in 50% of families. Dentinogenesis imperfecta is rare and may distinguish a subset of OI type I (formation of dentine). & Defects in COL1A1 are a cause of osteogenesis imperfecta type II (OI-II) [MIM:166210]; also known as osteogenesis imperfecta congenita. OI-II is lethal in the perinatal period and is charaterized by calvarial mineralization, beaded ribs, compressed femurs, marked long bone deformity and platyspondyly (congenital flattening of the vertebral bodies). & Defects in COL1A1 are a cause of osteogenesis imperfecta type III (OI-III) [MIM:259420]; also called progressively deforming osteogenesis imperfecta with normal sclerae. OI-III is characterized by progressively deforming bones, usually with moderate deformity at birth, sclerae is variable in color, dentinogenesis imperfecta and hearing loss are common. The stature is very short. & Defects in COL1A1 are a cause of osteogenesis imperfecta type IV (OI-IV) [MIM:166220]. OI-IV is charaterized by normal sclerae, moderate to mild deformity and variable short stature. Dentinogenesis imperfecta is common and hearing loss occurs in some patients. & Genetic variations in COL1A1 are associated with susceptibility to involutional osteoporosis [MIM:166710]; also known as senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mineral density, disrutption of bone microarchitecture, and the alteration of the amount and variety of non-collagenous proteins in bone. Osteoporotic bones are more at risk of fracture. & A chromosomal aberration involving COL1A1 is a cause of dermatofibrosarcoma protuberans (DFSP) [MIM:607907]. Translocation t(17;22)(q22;q13) with PDGF. DFSP is an uncommon, locally aggressive, but rarely metastasizing tumor of the deep dermis and subcutaneous tissue. It typically occurs during early or middle adult life and is most frequently located on the trunk and proximal extremities.
SIMILARITY: SwissProt: P02452 ## Belongs to the fibrillar collagen family. & Contains 1 VWFC domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB758 04053252581885

Documentation

Anti-Collagen Type I Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Collagen Type I Antibody Certificates of Analysis

TitleLot Number
GOAT ANTI-COLLAGEN TYPE I AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2145905 2145905
GOAT ANTI-COLLAGEN TYPE I AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2446724 2446724
GOAT ANTI-COLLAGEN TYPE I - 3152178 3152178
GOAT ANTI-COLLAGEN TYPE I - 3173835 3173835
GOAT ANTI-COLLAGEN TYPE I - 3257070 3257070
GOAT ANTI-COLLAGEN TYPE I - 3310708 3310708
GOAT ANTI-COLLAGEN TYPE I - 3318489 3318489
GOAT ANTI-COLLAGEN TYPE I - 3426865 3426865
GOAT ANTI-COLLAGEN TYPE I - 3468703 3468703
GOAT ANTI-COLLAGEN TYPE I - 3598005 3598005

References

Reference overviewPub Med ID
Differential regulation of cell functions by CSD peptide subdomains.
Reese, C; Dyer, S; Perry, B; Bonner, M; Oates, J; Hofbauer, A; Sessa, W; Bernatchez, P; Visconti, RP; Zhang, J; Hatfield, CM; Silver, RM; Hoffman, S; Tourkina, E
Respiratory research  14  90  2013

Show Abstract
24011378 24011378
Focal adhesion kinase modulates cell adhesion strengthening via integrin activation.
Michael, KE; Dumbauld, DW; Burns, KL; Hanks, SK; García, AJ
Molecular biology of the cell  20  2508-19  2009

Show Abstract Full Text Article
19297531 19297531
Arrangement of collagen fibers in human placental stem villi.
Leyla Sati,Ayse Yasemin Demir,Levent Sarikcioglu,Ramazan Demir
Acta histochemica  110  2008

Show Abstract
18275985 18275985
Effects of fixation and processing on the immunohistochemical visualization of type-I, -III and -IV collagen in paraffin-embedded liver tissue
Bedossa, P, et al
Histochemistry, 88:85-9 (1987)  1987

2449408 2449408

Data Sheet

Title
GOAT ANTI-COLLAGEN TYPE I AFFINITY PURIFIED POLYCLONAL ANTIBODY

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Alternative Format

Catalog Number Description
AB758B Anti-Collagen Type I Antibody, biotin conjugated

Product Families

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies