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204869 Anti-Complement 3 Goat pAb

This Anti-Complement 3 Goat pAb is validated for use in Flow Cytometry, Immunoblotting, Immunocytochemistry, Immunoelectrophoresis for the detection of Complement 3.

Anti-Complement 3 Goat pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
204869
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
Bab, Gp, Ht, Eq, H, M, Rb, RGtPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
204869-1ML Plastic ampoule 1 ml
Description
OverviewMonospecific for C3 complement component. May also recognize C3 fragments, C3a, C3b, and C3c.
Catalogue Number204869
Brand Family Calbiochem®
References
ReferencesInal, J.M., et al. 2002. J. Immunol. 168, 5213.
Williams, B.J., et al. 2001. Infect. Immun. 69, 695.
Hindmarsh, E.J., et al. 1998. J. Immunol. 160, 6128.
Product Information
FormLiquid
FormulationSerum diluted in 500 mM NaCl, 50 mM Tris-HCl, pH 7.5.
Positive controlPurified C3 protein (Cat. No. 204885)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Inal, J.M., et al. 2002. J. Immunol. 168, 5213. Williams, B.J., et al. 2001. Infect. Immun. 69, 695. Hindmarsh, E.J., et al. 1998. J. Immunol. 160, 6128. Flow Cytometry Williams, B.J., et al. 2001. Infect. Immun. 69, 695. Immunocytochemistry Hindmarsh, E.J., et al. 1998. J. Immunol. 160, 6128.
Key Applications Flow Cytometry
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoelectrophoresis
Application NotesFlow Cytometry (see application references)
Immunoblotting (see application references)
Immunocytochemistry (see application references)
Immunoelectrophoresis (use undiluted)
Application CommentsMonospecific to human C3 complement component. This antibody has also been reported to recognize the C3 fragments, C3a, C3b, and C3c. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified, human C3 complement component
ImmunogenHuman
HostGoat
IsotypeIgG
Species Reactivity
  • Baboon
  • Guinea Pig
  • Hamster
  • Horse
  • Human
  • Mouse
  • Rabbit
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
204869-1ML 04055977219654

Documentation

Anti-Complement 3 Goat pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Complement 3 Goat pAb Certificates of Analysis

TitleLot Number
204869

References

Reference overview
Inal, J.M., et al. 2002. J. Immunol. 168, 5213.
Williams, B.J., et al. 2001. Infect. Immun. 69, 695.
Hindmarsh, E.J., et al. 1998. J. Immunol. 160, 6128.

Citations

Title
  • Jameel M. Inal and Jurg A. Schifferli. (2002) Complement C2 Receptor Inhibitor Trispanning and the β-Chain of C4 Share a Binding Site for Complement C21. Journal of Immunology 168, 5213-5221.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision23-July-2007 RFH
    ApplicationFlow Cytometry (see application references)
    Immunoblotting (see application references)
    Immunocytochemistry (see application references)
    Immunoelectrophoresis (use undiluted)
    DescriptionGoat polyclonal antibody supplied as defibrinated, delipidized, and adsorbed (as needed) serum. Recognizes the C3 complement component.
    BackgroundThe complement system provides innate defense against microbial infection and is a "complement" to antibody mediated immunity. The complement system consists of thirty five interacting plasma and membrane associated proteins which contribute to host-defense and initiate and amplify inflammation, even in the preimmune state where specific antibodies and lymphocytes are not available. In addition to the complement components themselves, this system also contains several soluble factors that prevent spontaneous complement activation from occurring in solution, as well as, several regulatory membrane associated proteins that protect host cells from accidental complement attack. Cleavage of the C3 component by either the classical pathway or the alternative pathway releases C3a and C3b. C3a appears to be important in many inflammatory responses while the C3b fragment covalently binds to the cell or bacterial surface and plays a role in opsonisation. Binding of C3b to the C4b component of the C3 convertase, results in C5 convertase (C4b3b2a) formation.
    HostGoat
    Immunogen speciesHuman
    Immunogenpurified, human C3 complement component
    IsotypeIgG
    Speciesbaboon, not bovine, not canine, not chicken, not feline, guinea pig, hamster, horse, human, mouse, rabbit, rat, not sheep
    Positive controlPurified C3 protein (Cat. No. 204885)
    FormLiquid
    FormulationSerum diluted in 500 mM NaCl, 50 mM Tris-HCl, pH 7.5.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsMonospecific to human C3 complement component. This antibody has also been reported to recognize the C3 fragments, C3a, C3b, and C3c. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesInal, J.M., et al. 2002. J. Immunol. 168, 5213.
    Williams, B.J., et al. 2001. Infect. Immun. 69, 695.
    Hindmarsh, E.J., et al. 1998. J. Immunol. 160, 6128.
    Citation
  • Jameel M. Inal and Jurg A. Schifferli. (2002) Complement C2 Receptor Inhibitor Trispanning and the β-Chain of C4 Share a Binding Site for Complement C21. Journal of Immunology 168, 5213-5221.
    		•
    Application referencesImmunoblotting Inal, J.M., et al. 2002. J. Immunol. 168, 5213. Williams, B.J., et al. 2001. Infect. Immun. 69, 695. Hindmarsh, E.J., et al. 1998. J. Immunol. 160, 6128. Flow Cytometry Williams, B.J., et al. 2001. Infect. Immun. 69, 695. Immunocytochemistry Hindmarsh, E.J., et al. 1998. J. Immunol. 160, 6128.