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ABN302 Anti-Complex I-75kD Antibody

Anti-Complex I-75kD, Cat. No. ABN302, is a rabbit polyclonal antibody that detects Complex I-75kD and is tested for use in Western Blotting and Immunohistochemistry.

Anti-Complex I-75kD Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: NDUFS1 UniProt Number: P28331
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ABN302
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN302
DescriptionAnti-Complex I-75kD Antibody
Alternate Names
  • NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
  • Complex I-75kD
  • CI-75kD
Background InformationComplex I-75kD (CI-75kD) is also called NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1). Complex I-75kD is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which transfers electrons from NADH to the respiratory chain, and is thought to be part of the minimal assembly required for catalysis. Complex I-75kD defects are thought to be a cause of Mitochondrial complex I deficiency (MT-C1D).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-Complex I-75kD, Cat. No. ABN302, is a rabbit polyclonal antibody that detects Complex I-75kD and is tested for use in Western Blotting and Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesTested Applications

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Complex I-75kD in rat brain, human brain, and human heart tissue lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Complex I-75kD in human kidney and human heart tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Complex I-75kD.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine, Chimpanzee based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NDUFS1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight75 kDa observed. 79.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Complex I-75kD in mouse brain tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABN302 04053252923302

Documentation

Anti-Complex I-75kD Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Complex I-75kD Antibody Certificates of Analysis

TitleLot Number
Anti-Complex I-75kD 3077402
Anti-Complex I-75kD - 3521078 3521078
Anti-Complex I-75kD - 3589768 3589768
Anti-Complex I-75kD - 3839156 3839156
Anti-Complex I-75kD - 4090117 4090117
Anti-Complex I-75kD - Q2269474 Q2269474
Anti-Complex I-75kD -2816644 2816644
Anti-Complex I-75kD -2835268 2835268
Anti-Complex I-75kD Polyclonal Antibody 3147028
Anti-Complex I-75kD Polyclonal Antibody 3042003
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