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MABT903 Anti-Connexin 43 Antibody, N-terminal Antibody, clone P1E11.B19

This mouse monoclonal Anti-Connexin 43, N-terminal, clone P1E11.B19, Cat. No. MABT903 is tested for use in Immunocytochemistry and Western Blotting for the detection of Connexin-43.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Gja1, Cx43 UniProt Number: P08050
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MABT903
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT903
DescriptionAnti-Connexin 43 Antibody, N-terminal Antibody, clone P1E11.B19
Alternate Names
  • Gap junction alpha-1 protein
  • CX43
  • Gap junction 43 kDa heart protein
Background InformationGap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-Connexin 43, N-terminal, clone P1E11.B19, Cat. No. MABT903 is tested for use in Immunocytochemistry and Western Blotting for the detection of Connexin-43.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Sosinsky, G.E., et. al. (2007). Biochem. 408(3):375-85).

Western Blotting Analysis (WB): A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampe s Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).

Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Kalcheva, N., et. al. (2007). Proc Natl Acad Sci USA. 104(51):20512-6).

Western Blotting Analysis: 1-4 µg/mL from a representative lot detected Connexin 43 in 10 µg of rat and mouse heart tissue lysates.

Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Gellhaus, A., et. al. (2004). J Biol Chem. 279(35):36931-42).

Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Goldberg, G.S., et. al. (2002). J Biol Chem. 277(39):36725-30).

Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Solan, J.L., et. al. (2003). J Cell Sci. 116(Pt 11):2203-11).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the first 20 amino acids from the N-terminal region of rat connection-43.
EpitopeN-terminus
CloneP1E11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone P1E11.B19 detects connexin-43 in most mammalian species. It targets an epitope with in the first 20 amino acids from the N-terminal end.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine, Canine, Chicken, Hamster, Horse, Porcine, Rabbit, and Rhesus Macaque based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Gja1
  • Cx43
Purification MethodProtein G purified
UniProt Number
Molecular Weight~43 kDa observed; 43.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human heart tissue lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected Connexin 43 in 10 µg of human heart tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT903 04054839199363

Documentation

Anti-Connexin 43 Antibody, N-terminal Antibody, clone P1E11.B19 Certificates of Analysis

TitleLot Number
Anti-Connexin 43, N-terminal, clone P1E11.B19 3083524
Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3198419 3198419
Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3666810 3666810
Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3804845 3804845
Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3883668 3883668
Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3888842 3888842
Anti-Connexin 43, N-terminal, clone P1E11.B19 - 3980287 3980287
Anti-Connexin 43, N-terminal, clone P1E11.B19 - 4107729 4107729
Anti-Connexin 43, N-terminal, clone P1E11.B19 Monoclonal Antibody Q2841560