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CC12 Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)

This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bcl-1, Anti-PRAD-1

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CC12
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
CC12-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.
Catalogue NumberCC12
Brand Family Calbiochem®
SynonymsAnti-Bcl-1, Anti-PRAD-1
References
ReferencesInaba, T., et al. 1992. Genomics 13, 565.
Jiang, W., et al. 1992. Cancer Res. 2, 2980.
Pines, J. 1992. Cell Growth Differ. 2, 305.
Xiong, Y., et al. 1992. Genomics 13, 575.
Xiong, Y., et al. 1992. Cell 71, 504.
Motokura, T., et al. 1991. Nature 350, 512.
Solomon, M.J., et al. 1990. Cell 63, 1013.
Draetta, G., and Beach, D. 1988. Cell 54, 17.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controltrp E (Ab-1)
Positive controlMCF7, or A431 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting, Immunoprecipitation, and Neutralization Studies Lukas, J., et al. 1994. Oncogene 9, 707. Immunofluorescence and Flow Cytometry Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Lukas, J., et al. 1994. Oncogene 9, 707. Paraffin and Frozen Sections Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Gillett, C., et al. 1994. Cancer Res. 54, 1812-1817.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Neutralization Studies
Paraffin Sections
Application NotesFlow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)
Frozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)
Immunoprecipitation (see application references)
Neutralization Studies (see application references)
Paraffin Sections (5 µg/ml, heat pre-treatment required)
Application CommentsDoes not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human cyclin D1
ImmunogenHuman
CloneDCS-6
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
CC12-100UG 04055977228465

Documentation

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) Certificates of Analysis

TitleLot Number
CC12

References

Reference overview
Inaba, T., et al. 1992. Genomics 13, 565.
Jiang, W., et al. 1992. Cancer Res. 2, 2980.
Pines, J. 1992. Cell Growth Differ. 2, 305.
Xiong, Y., et al. 1992. Genomics 13, 575.
Xiong, Y., et al. 1992. Cell 71, 504.
Motokura, T., et al. 1991. Nature 350, 512.
Solomon, M.J., et al. 1990. Cell 63, 1013.
Draetta, G., and Beach, D. 1988. Cell 54, 17.

Citations

Title
  • Takeshi Shimamura, et al. (2005) Epidermal growth factor receptors harboring kinase domain mutations associate with the heat shock protein 90 chaperone and are destabilized following exposure to geldanamycins. Cancer Research 65, 6401-6408.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision01-August-2007 RFH
    SynonymsAnti-Bcl-1, Anti-PRAD-1
    ApplicationFlow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)
    Frozen Sections (see application references)
    Immunoblotting (1 µg/ml)
    Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)
    Immunoprecipitation (see application references)
    Neutralization Studies (see application references)
    Paraffin Sections (5 µg/ml, heat pre-treatment required)
    DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.
    BackgroundCyclins are a family of proteins implicated in the induction and control of mitosis. They are characterized by their accumulation and dramatic destruction in a cell cycle dependent manner. Cyclins are necessary for cdc/cdk kinase activity and are one of the subunits of the active kinase, in association with cdc2 or one of the cdk family members. Several different major cyclin families have been identified to date. These include the A, B, D and E family of cyclins. The D family of cyclins has been associated with a wide variety of proliferative diseases but their biochemical role is still unknown. The D cyclins appear in early G1, before the B cyclins. The human cyclin D1 gene has been mapped to the 11q13 locus and has been shown to be the PRAD1 gene product. PRAD1 was originally identified as being a clonally rearranged and overexpressed gene linked to the bcl-1 locus in a subset of benign parathyroid tumors. Amplification of cyclin D1 genes has also been demonstrated in human esophageal cancer.
    HostMouse
    Immunogen speciesHuman
    Immunogenrecombinant human cyclin D1
    CloneDCS-6
    IsotypeIgG2a
    Specieshuman, mouse, rat
    Positive controlMCF7, or A431 cells or breast carcinoma tissue
    Negative controltrp E (Ab-1)
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsDoes not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesInaba, T., et al. 1992. Genomics 13, 565.
    Jiang, W., et al. 1992. Cancer Res. 2, 2980.
    Pines, J. 1992. Cell Growth Differ. 2, 305.
    Xiong, Y., et al. 1992. Genomics 13, 575.
    Xiong, Y., et al. 1992. Cell 71, 504.
    Motokura, T., et al. 1991. Nature 350, 512.
    Solomon, M.J., et al. 1990. Cell 63, 1013.
    Draetta, G., and Beach, D. 1988. Cell 54, 17.
    Citation
  • Takeshi Shimamura, et al. (2005) Epidermal growth factor receptors harboring kinase domain mutations associate with the heat shock protein 90 chaperone and are destabilized following exposure to geldanamycins. Cancer Research 65, 6401-6408.
    		•
    Application referencesImmunoblotting, Immunoprecipitation, and Neutralization Studies Lukas, J., et al. 1994. Oncogene 9, 707. Immunofluorescence and Flow Cytometry Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Lukas, J., et al. 1994. Oncogene 9, 707. Paraffin and Frozen Sections Bartkova, J., et al. 1995. Cancer Res. 55, 949-956. Bartkova, J., et al. 1994. J. Pathol. 172, 237-245. Gillett, C., et al. 1994. Cancer Res. 54, 1812-1817.