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MABE1988-100UG Anti-Cyclin D2 Antibody, clone D2-34B1-3

Anti-Cyclin D2, clone D2-34B1-3, Cat. No. MABE1988, is a rat monoclonal antibody that detects G1/S-specific cyclin-D2 and is tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

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MABE1988-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABE1988-100UG
DescriptionAnti-Cyclin D2 Antibody, clone D2-34B1-3
Alternate Names
  • G1/S-specific cyclin-D2
  • CCND2
Background InformationG1/S-specific cyclin-D2 (UniProt: P30280; also known and Cyclin D2;CCND2) is encoded by the Ccnd2 (also known as Cyl-2) gene (Gene ID: 12444) in murine species. Cell cycle progression is regulated by the sequential events that include activation and subsequent inactivation of cyclin dependent kinases (Cdk) and cyclins. Cyclins impart substrate specificity to the CDK/cyclin complex. D-type cyclins (D1, D2, and D3) are known to have critical roles in cell cycle progression, differentiation, and malignant transformation. They are encoded by distinct genes but show significant amino-acid similarity. D-type cyclins are shown to associate with cyclin-dependent kinase 4 and 6 (CDK4, CDK6) and promote phosphorylation and subsequent inactivation of the retinoblastoma tumor suppressor gene product, RB and RB-related proteins. Phosphorylation of RB allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes that are responsible for the progression through the G1 phase.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Cyclin D2, clone D2-34B1-3, Cat. No. MABE1988, is a rat monoclonal antibody that detects G1/S-specific cyclin-D2 and is tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunofluorescence
  • Immunohistochemistry
Application NotesTested Applications

Immunofluorescence Analysis: A representative lot detected Cyclin D2 in Immunofluorescence applications (Vallance, S.J., et al. (1994). Hybridoma.;13(1):37-44).

Western Blotting Analysis: A representative lot detected Cyclin D2 in Western Blotting applications (Vallance, S.J., et al. (1994). Hybridoma.;13(1):37-44).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Cyclin D2 in Immunoprecipitation applications (Vallance, S.J., et al. (1994). Hybridoma.;13(1):37-44).

Immunohistochemistry Applications: A representative lot detected Cyclin D2 in Immunohistochemistry applications (Rowan, S., et al. (2008). Dev Biol.;321(1):111-22).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant murine Cyclin D2.
EpitopeUnknown
CloneD2-34B1-3
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone D2-34B1-3 is a rat monoclonal antibody that detects Cyclin D2.
IsotypeIgG2a
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CCnd2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~28 kDa observed; 32.90 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse spleen tissue lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cyclin D2 in Mouse spleen tissue lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1988-100UG 04065266791457