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MABT845-25UG Anti-Cytokeratin-20 Antibody, clone XQ1

Anti-Cytokeratin-20, clone XQ1, Cat. No. MABT845, is a mouse monoclonal antibody that detects Keratin, type I cytoskeletal 20 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-Cytokeratin-20 Antibody, clone XQ1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Krt20 UniProt Number: Q9D312
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MABT845-25UG
25 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIF, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT845-25UG
DescriptionAnti-Cytokeratin-20 Antibody, clone XQ1
Alternate Names
  • Keratin
  • type I cytoskeletal 20
  • CK-20
  • Keratin-20
  • K20
Background InformationKeratin, type I cytoskeletal 20 (UniProt: Q9D312; also known as Cytokeratin-20, CK-20, Keratin-20, K20) is encoded by the Krt20 gene (Gene ID: 66809) in murine species. Cytokeratin-20 is type I keratin of the intermediate filament family and is recognized as a marker of intestinal differentiation. It is expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Cytokeratin-20 is also an excellent marker for certain types of carcinomas, such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. It plays a significant role in maintaining keratin filament organization in intestinal epithelia and when phosphorylated, it also plays a role in the secretion of mucin in the small intestine. Cytokeratin-20 can undergo hyperphosphorylation at serine 13 during the early stages of apoptosis, which becomes less prominent during the later stages. Phosphorylation at serine 13 is shown to increase in response to stress brought on by cell injury. During apoptosis it is cleaved at Asp 235 by caspases. (Ref.: Moll, R., et al. (1992). Am. J. Pathol. 140(2); 427-447).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Applications
ApplicationAnti-Cytokeratin-20, clone XQ1, Cat. No. MABT845, is a mouse monoclonal antibody that detects Keratin, type I cytoskeletal 20 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunofluorescence Analysis: A representative lot detected Cytokeratin-20 in Immunofluorescence applications (Zhou, Q., et. al. (2003). Mol Biol Cell. 14(7):2959-71).

Western Blotting Analysis: A representative lot detected Cytokeratin-20 in Western Blotting applications (Zhou, Q., et. al. (2003). Mol Biol Cell. 14(7):2959-71).
Biological Information
ImmunogenA full-length purified murine Cytokeratin-20.
CloneXQ1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone XQ1 is a mouse monoclonal antibody that detects cytoketatin-20 in murine tissues.
IsotypeIgMκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Does not react with human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Krt20
Purification MethodPurified by ion-exchange chromatography
UniProt Number
Molecular Weight49.03 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Cytokeratin-20 in mouse colon tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABT845-25UG 04054839563379