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MABE967 Anti-DNA polymerase delta p125 Antibody, clone 11E10.1

Anti-DNA polymerase delta p125 Antibody, clone 11E10.1 is an antibody against DNA polymerase delta p125 Antibody for use in Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: POLD1, POLD UniProt Number: P28340
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MABE967
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE967
DescriptionAnti-DNA polymerase delta p125 Antibody, clone 11E10.1
Alternate Names
  • DNA polymerase delta catalytic subunit
  • DNA polymerase delta p125
  • DNA polymerase subunit delta p125
Background InformationDNA polymerase delta catalytic subunit (EC 2.7.7.7; UniProt P28340; also known as CDC2 homolog, DNA polymerase subunit delta p125) is encoded by the POLD1 (also known as CDC2, MDPL, POLD, CRCS10) gene (Gene ID 5424) in human. DNA replication in eukaryotes involves multiple enzymes including the B family polymerases Pol α, Pol δ, and Pol ɛ. While Pol α initiates DNA synthesis, Pol δ and Pol ɛ replace Pol α after primer extension and perform the bulk of DNA replication. Pol δ and Pol ɛ are heterotetramers in higher eukaryotes comprised of POLD1 and POLE as the respective catalytic subunit, as well as accessory subunits (POLD2/3/4 and POLE2/3/4) that interact with cofactors such as Proliferating Cell Nuclear Antigen (PCNA). Both POLD1 and POLE contain an exonuclease domain (a.a. 268–471 of POLE and 304–517 of POLD1) that proof-reading capability during DNA replication. In addition to DNA synthesis, Pol ɛ and Pol δ are believed to participate in chromosomal DNA repairs, including nucleotide excision repair (NER), ismatch repair (MMR) and repair of double strand breaks (DSBR). Germline exonuclease domain mutations (EDMs) in POLD1 and POLE cause polymerase proofreading-associated polyposis (PPAP). Tumors with EDMs exhibits dramatically increased base substitutions.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-DNA polymerase delta p125 Antibody, clone 11E10.1 is an antibody against DNA polymerase delta p125 Antibody for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected DNA polymerase delta p125 in 10 µg of Ramos cell lysate.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of human DNA polymerase delta p125.
EpitopeN-terminus
Clone11E10.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • POLD1
  • POLD
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~125 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected DNA polymerase delta p125 in 10 µg of Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE967 04055977294743

Documentation

Anti-DNA polymerase delta p125 Antibody, clone 11E10.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-DNA polymerase delta p125 Antibody, clone 11E10.1 Certificates of Analysis

TitleLot Number
Anti-DNA polymerase delta p125, clone 11E10.1 - 3517397 3517397
Anti-DNA polymerase delta p125, clone 11E10.1 - 3921470 3921470
Anti-DNA polymerase delta p125, clone 11E10.1 -Q2611625 Q2611625

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Posters

Title
Post-Translational Modifications