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MABC1594-25UL Anti-DR6 (CD358) Antibody, clone 6B6

Anti-DR6 (CD358), clone 6B6, Cat. No. MABC1594, is a rat monoclonal antibody that detects Death Receptor 6 (DR6) and is tested for use in Western Blotting.

MABC1594-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABC1594-25UL
DescriptionAnti-DR6 (CD358) Antibody, clone 6B6
Alternate Names
  • Tumor necrosis factor receptor superfamily member 21
  • Death receptor 6
  • CD358
Background InformationTumor necrosis factor receptor superfamily member 21 (UniProt: Q9EPU5; also known as Death receptor 6, DR6, CD358) is encoded by the Tnfrsf21 (also known as Dr6) gene (Gene ID: 94185) in murine species. DR6 is a single-pass type I membrane glycoprotein that is an orphan member of the TNF receptor superfamily. It controls cell death and differentiation in a cell autonomous manner in different cell types. It is ubiquitous expressed and higher expression has been observed in adult spleen, thymus, testis, prostate, ovary, small intestine, colon, brain, lung and kidney tissue. It is also detected in fetal brain, liver and lung. DR6 is expressed at low levels in proliferating progenitors in the spinal cord, but it is highly expressed by differentiating neurons within the spinal cord and adjacent dorsal root ganglia. Expressed by developing neurons as they differentiate and enter a pro-apoptotic state. DR6 promotes apoptosis, possibly via a pathway that involves the activation of NF-kappa-B and also promote apoptosis mediated by BAX and by the release of cytochrome c from the mitochondria into the cytoplasm. It also participates in neuronal apoptosis in response amyloid peptides derived from amyloid precursor protein. DR6 is reported to be a substrate for ADAM10 and -secretase. DR6 is shown to be involved in peripheral nervous system (PNS) myelination and DR6 knockout mice display precocious myelination in the PNS. DR6 is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 42-349), a transmembrane domain (aa 350-370), and a cytoplasmic domain (aa 371-655). It contains four TNFR-Cys repeats and its death domain is localized to amino acids 415-498. (Ref.: Columbo, A., et al. (2018). EMBO J. 37: e97390).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-DR6 (CD358), clone 6B6, Cat. No. MABC1594, is a rat monoclonal antibody that detects Death Receptor 6 (DR6) and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected DR6 (CD358) in Western Blotting applications (Colombo, A., et. al (2018. EMBO J. 37(7)).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Mouse brain tissue lysates from wild type (WT) versus DR6 knock-out (KO) (Courtesy of Dr. Hung-en Hsia, Group of Neuroproteomics (Head: Prof. Dr. Stefan Lichtenthaler), German Center for Neurodegenerative Diseses (DZNE).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRecombinant fragment corresponding to full-length ectodomain region of mouse DR6 with an N‐terminal CD5 signal peptide and a C‐terminal 2XStrepII tag.
Epitopeextracellular domain
Clone6B6
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 6B6 is a rat monoclonal antibody that detects Death Receptor 6. It targets an epitope within the extracellular domain.
IsotypeIgG2b
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tnfrsf21
  • Dr6
Purification MethodProtein G purified
UniProt Number
Molecular Weight71.98 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity confirmation by isotyping test.



Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC1594-25UL 04061842414727

Documentation

Anti-DR6 (CD358) Antibody, clone 6B6 SDS

Title

Safety Data Sheet (SDS) 

Anti-DR6 (CD358) Antibody, clone 6B6 Certificates of Analysis

TitleLot Number
Anti-DR6 (CD358), clone 6B6 - Q3490923 Q3490923

References

Reference overviewPub Med ID
Non-cell-autonomous function of DR6 in Schwann cell proliferation
Alessio Colombo 1 , Hung-En Hsia 1 2 , Mengzhe Wang 3 , Peer-Hendrik Kuhn 2 , Monika S Brill 3 , Paolo Canevazzi 4 , Regina Feederle 1 5 6 , Carla Taveggia 4 , Thomas Misgeld 1 3 6 7 , Stefan F Lichtenthaler
EMBO J  37(7)  e97390  2018

Show Abstract 		29459438 29459438