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MABS1154 Anti-Derlin-1 Antibody, clone 8G11.2

This Anti-Derlin-1 Antibody, clone 8G11.2 is validated for use in Western Blotting, Immunocytochemistry for the detection of Derlin-1.

Anti-Derlin-1 Antibody, clone 8G11.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: DERL1, DER1, UNQ243/PRO276 UniProt Number: Q9BUN8
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MABS1154
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1154
DescriptionAnti-Derlin-1 Antibody, clone 8G11.2
Alternate Names
  • Degradation in endoplasmic reticulum protein 1
  • Der1-like protein 1
  • Derlin-1
  • DERtrin-1
Background InformationDerlin-1 (UniProt: Q9BUN8; also known as Degradation in endoplasmic reticulum protein 1, DERtrin-1, Der1-like protein 1) is encoded by the DERL1 (also known as DER1, UNQ243/PRO276) gene (Gene ID: 79139) in human. Derlin-1 is a multi-pass membrane protein that serves as an E3 ligase mediator that links target proteins to ubiquitin-mediated proteasomal degradation in the cytosol. It is a functional sub-unit of the ER-associated degradation (ERAD) that forms a retro-translocation channel in the ER membrane and functions in the recognition of misfolded membrane or transmembrane proteins and their subsequent degradation in the ubiquitin-proteasomal system. Before any ERAD proteins are processed by the cytoplasmic ubiquitin-proteasome system, they are translocated across the ER membrane through an AAA ATPase p97/VCP-driven process. Derlin-1 is also involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Derlin-1 can form homo- and heterooligomers with Derlin-2 and -3, however, binding to Derlin-3 is shown to be DERL3 is lower. Derlin-1 contains three cytoplasmic domains (aa 2-22, 81-105, and 176-251), four transmembrane domains (aa 23-43, 60-80, 106-126, 155-175), and two lumenal domains (aa 44-59 and 127-154). (Ref.: You, H., et al. (2017). J. Cell Sci. 130(6); 1027-1036).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Derlin-1 Antibody, clone 8G11.2 is validated for use in Western Blotting, Immunocytochemistry for the detection of Derlin-1.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: An 1:50 dilution from a representative lot detected Derlin-1 in HUVECs, A431 and HeLa cells.
Biological Information
ImmunogenGST-tagged recombinant human derlin-1 C-terminal cytoplasmic fragment.
EpitopeC-terminal cytoplasmic domain.
Clone8G11.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with both spliced isoforms of human Derlin-1 reported by UniProt (Q9BUN8).
IsotypeIgG2bκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DERL1
  • DER1
  • UNQ243/PRO276
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~24 kDa observed. 28.80 kDa (isoform 1) and 26.42 kDa (isoform 2) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Derlin-1 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS1154 04055977331202