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DR1095 Anti-E2F2 Rabbit pAb

Anti-E2F2, rabbit polyclonal, recognizes the ~40-45 kDa E2F2 protein in HeLa cells and in mouse kidney. It is validated for use in Western blotting, and immunocytochemistry.

Anti-E2F2 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-E2F Transcription Factor 2

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DR1095
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
DR1095-100UG 100 μg
Description
OverviewRecognizes the ~40-45 kDa E2F2 protein in HeLa cells and mouse kidney tissue.
Catalogue NumberDR1095
Brand Family Calbiochem®
SynonymsAnti-E2F Transcription Factor 2
Application Data
Detection of mouse E2F2 by immunoblotting. Sample: Whole tissue extract from mouse kidney. Primary antibody: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human E2F2 by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1:1200) and an Anti-E2F2 Mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlMouse kidney tissue, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-5 µg/ml)
Immunocytochemistry (1:1200)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, human E2F2 (aa 1-437)
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
DR1095-100UG 04055977226140

Documentation

Anti-E2F2 Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-E2F Transcription Factor 2
ApplicationImmunoblotting (1-5 µg/ml)
Immunocytochemistry (1:1200)
Application Data
Detection of mouse E2F2 by immunoblotting. Sample: Whole tissue extract from mouse kidney. Primary antibody: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human E2F2 by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1:1200) and an Anti-E2F2 Mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~40-45 kDa E2F2 protein.
BackgroundThe E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds specifically to retinoblastoma protein in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.
HostRabbit
Immunogen speciesHuman
ImmunogenFull-length, human E2F2 (aa 1-437)
IsotypeIgG
Specieshuman, mouse
Positive controlMouse kidney tissue, HeLa cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review