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MABE945 Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8

This Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8 is validated for use in Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and Western Blotting for the detection of E3 ubiquitin-protein ligase UHRF1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: UHRF1, ICBP90, NP95, RNF106 UniProt Number: Q96T88
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MABE945
200 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, ChIP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE945
DescriptionAnti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8
Alternate Names
  • E3 ubiquitin-protein ligase UHRF1
  • Inverted CCAAT box-binding protein of 90 kDa
  • Nuclear protein 95
  • Nuclear zinc finger protein Np95
  • HuNp95
  • hNp95
  • RING finger protein 106
  • Transcription factor ICBP90
  • Ubiquitin-like PHD and RING finger domain-containing protein 1
  • hUHRF1
  • Ubiquitin-like-containing PHD and RING finger domains protein 1
Background InformationUbiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-E3 ubiquitin-protein ligase UHRF1 Antibody, clone 7C8 is validated for use in Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and Western Blotting for the detection of E3 ubiquitin-protein ligase UHRF1.
Key Applications
  • Immunocytochemistry
  • Chromatin Immunoprecipitation (ChIP)
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected E3 ubiquitin-protein ligase UHRF1 in overexpressed lysate (Martin Walsh, Mount Sinai School of Medicine).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated E3 ubiquitin-protein ligase UHRF1 in NTera2 cells (Martin Walsh, Mount Sinai School of Medicine).
Biological Information
ImmunogenRecombinant protein corresponding to human E3 ubiquitin-protein ligase UHRF1.
Clone7C8
Concentration1 mg/mL
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • UHRF1
  • ICBP90
  • NP95
  • RNF106
Purification MethodProtein G Purified
UniProt Number
Molecular Weight90 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected E3 ubiquitin-protein ligase UHRF1 in HeLa cells.
Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE945 04053252992865