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MABE1989-100UL Anti-EAF1 Antibody, clone 1E6

Anti-EAF1, clone 1E6, Cat. No. MABE1989, is a mouse monoclonal antibody that detects ELL-associated factor 1 and is tested for use in Immunocytochemistry, Immunoprecipitation, Northern Blotting, and Western Blotting.

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MABE1989-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABE1989-100UL
DescriptionAnti-EAF1 Antibody, clone 1E6
Alternate Names
  • ELL-associated factor 1
Background InformationELL-associated factor 1 (UniProt: Q96JC9; also known as EAF1) is encoded by the EAF1 gene (Gene ID: 85403) in human. EAF1, a member of the EAF family, is a component of the super elongation complex that is composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF, the P-TEFb complex and ELL. It acts as a transcriptional transactivator of ELL and ELL2 elongation activities. In its C-terminal region resides a sequence (aa 182-262) that is shown to be essential for its transactivation activity. The carboxy-terminal of ELL contains a sequence (aa 508-621) that is shown to be essential for interaction with EAF1. Both EAF1 and EAF2 are shown to play important roles in tumor suppression and embryogenesis. They are also reported to inhibit Wnt/β-catenin signaling, thereby modulating mesodermal and neural patterning in the embryo and their ectopic expression in embryos and cultured cells can block β-catenin reporter activity. They interact with the β-catenin center region containing 12 Armadillo repeats and the C-terminus region. EAF1 is strongly expressed in many tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, and colon. Two isoforms of EAF1 have been described that are produced by alternative splicing. (Ref.: Liu, JX., et al. (2013). Development. 140(5); 1067-1078; Simone, F., et al. (2003). Blood. 101(6); 2355-2362; Simone, F., et al. (2001); Blood 98(1); 201-209).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-EAF1, clone 1E6, Cat. No. MABE1989, is a mouse monoclonal antibody that detects ELL-associated factor 1 and is tested for use in Immunocytochemistry, Immunoprecipitation, Northern Blotting, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Northern Blotting
  • Western Blotting
  • Immunoprecipitation
Application NotesTested Applications

Immunocytochemistry Analysis: A representative lot detected EAF1 in Immunocytochemistry applications (Simone, F., et al. (2001). Blood.;98(1):201-9).

Immunoprecipitation Analysis: A representative lot immunoprecipitated EAF1 in Immunoprecipitation applications (Simone, F., et al. (2001). Blood.;98(1):201-9; Simone, F., et al. (2003). Blood.;101(6):2355-62).

Western Blotting Analysis: A representative lot detected EAF1 in Western Blotting applications (Simone, F., et al. (2001). Blood.;98(1):201-9).

Northern Blotting: A representative lot detected EAF1 in Northern Blotting applications (Simone, F., et al. (2001). Blood.;98(1):201-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged full-length recombinant human ELL-associated factor 1 (EAF1).
EpitopeUnknown
Clone1E6
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1F6 is a mouse monoclonal antibody that detects human ELL-associated factor 1 (EAF1).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EAF1
Purification MethodProtein G purified
UniProt Number
Molecular Weight29.04 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected EAF1 in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1989-100UL 04065266908091