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GR13L Anti-EGFR (Ab-3) Mouse mAb (225)

Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.

Anti-EGFR (Ab-3) Mouse mAb (225) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-EGF-R

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GR13L
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
GR13L-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.
Catalogue NumberGR13L
Brand Family Calbiochem®
SynonymsAnti-EGF-R
References
ReferencesHunter T. 1984. Nature 311, 414.
Reynolds, F.H., et. al 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
Product Information
FormLyophilized
FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
Negative controlHL-60 cells
Positive controlA431 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511.
Key Applications Immunoprecipitation
Neutralization Studies
Application NotesImmunoprecipitation (1-2 µg per sample)
Neutralization Studies (2 µg/ml, see comments and application references)
Application CommentsThis antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpartially purified, human EGFR from A431 cells
ImmunogenHuman
Clone225
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
GR13L-100UG 04055977228236

Documentation

Anti-EGFR (Ab-3) Mouse mAb (225) SDS

Title

Safety Data Sheet (SDS) 

Anti-EGFR (Ab-3) Mouse mAb (225) Certificates of Analysis

TitleLot Number
GR13L

References

Reference overview
Hunter T. 1984. Nature 311, 414.
Reynolds, F.H., et. al 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-October-2007 JSW
SynonymsAnti-EGF-R
ApplicationImmunoprecipitation (1-2 µg per sample)
Neutralization Studies (2 µg/ml, see comments and application references)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
BackgroundEpidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF receptor is phosphorylated on tyrosine residues. Subsequent to binding, EGF receptor complexes are internalized and cell proliferation takes place with partially-purified EGF receptor from human A431 epidermal carcinoma cells.
HostMouse
Immunogen speciesHuman
Immunogenpartially purified, human EGFR from A431 cells
Clone225
IsotypeIgG₁
Specieshuman, not mouse, not rat
Positive controlA431 cells
Negative controlHL-60 cells
FormLyophilized
FormulationLyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
PreservativeNone
CommentsThis antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesHunter T. 1984. Nature 311, 414.
Reynolds, F.H., et. al 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
Application referencesOriginal Clone Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7755. Kawamoto, T., et al. 1984. J. Biol. Chem. 259, 7761. Masui, H., et al. 1984. Cancer Res. 44, 1002. Kawamoto, T., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Biol. Med. 1, 511.