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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABC1023 Sigma-AldrichAnti-EGR1 Antibody, clone 16A10.1

Anti-EGR1 Antibody, clone 16A10.1 is an antibody against EGR1 for use in Western Blotting, Immunocytochemistry.

Anti-EGR1 Antibody, clone 16A10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Apoptosis & Cancer Research Sub-Category: Transcription Factors Gene Symbol: EGR1, KROX24, ZNF225 UniProt Number: P18146

Replacement Information

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABC1023
Description	Anti-EGR1 Antibody, clone 16A10.1
Alternate Names	Early growth response protein 1 AT225 EGR-1 Nerve growth factor-induced protein A NGFI-A Transcription factor ETR103 Transcription factor Zif268 Zinc finger protein 225 Zinc finger protein Krox-24
Background Information	Early growth response protein 1 (UniProt P18146; also known as AT225, EGR-1, Nerve growth factor-induced protein A, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24) is encoded by the EGR1 (also known as KROX24, ZNF225) gene (Gene ID 1958) in human. EGR-1 is the founding member of the early growth response (EGR) family of Cys2-His2-type zinc finger transcription factors that share a high homology in their DNA-binding region. EGR-1 is ubiquitously expressed in many cell types and binds to a consensus DNA motif (GCGGTGGGCG) in target genes, including c-Myc, cyclins D2 & G2, p19, TNF, IL-2, CD44, and ICAM-1. EGR1 is known as an immediate-early response gene due to the rapid kinetics of its induction by various signals, including growth factors, cytokines, and stress. Human EGR-1 is a 543-amino acid protein with three C2H2-type Zinc fingers (a.a. 338-362, 368-390, 396-418).

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-EGR1 Antibody, clone 16A10.1 is an antibody against EGR1 for use in Western Blotting, Immunocytochemistry.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: An 1:250 dilution of this antibody from a representative lot detected EGF-induced upregulation of nuclear EGR1 immunoreactivity in serum-starved HeLa and NIH/3T3 cells.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to an internal fragment of human EGR1.
Epitope	Internal.
Clone	16A10.1
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001955
Gene Symbol	EGR1 KROX24 ZNF225
Purification Method	Protein G Purified
UniProt Number	P18146
Molecular Weight	~80 kDa observed. 57.51 kDa calculated. The observed target band size is consistent with the reported apparent molecular weight (Krones-Herzig, A., et al. (2005). Cancer Res. 65(12):5133-5143; Josefsen, K., et al. (1999). Diabetologia. 42(2):195-203).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HUVEC lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected VEGF-induced EGR1 expression in serum-starved HUVECs.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
MABC1023	04055977330212

Title	Lot Number
Anti-EGR1, clone 16A10.1 - 3433050	3433050
Anti-EGR1, clone 16A10.1 -Q2659403	Q2659403

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