Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABE362 Anti-EZH2 Antibody, clone AE25

This Anti-EZH2 Antibody, clone AE25 is validated for use in Western Blotting, IHC for the detection of EZH2.

Anti-EZH2 Antibody, clone AE25 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: ENX-1, KMT6 UniProt Number: Q15910
View Products on Sigmaaldrich.com
MABE362
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE362
Replaces04-1047
DescriptionAnti-EZH2 Antibody, clone AE25
Alternate Names
  • Enhancer of zeste homolog 2
  • Lysine N-methyltransferase 6
  • enhancer of zeste 2
  • histone-lysine N-methyltransferase EZH2
Background InformationEZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis, and placenta. EZH2 and BMI-1 genes are coexpressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene.
References
Product Information
FormatPurified
Control
  • T47D cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-EZH2 Antibody, clone AE25 is validated for use in Western Blotting, IHC for the detection of EZH2.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blot Analysis: A 1:500 dilution from a representative lot detected EZH2 in Jurkat and HeLa cell lysates.

Immunohistochemistry Analysis: A representative lot detected EZH2 in breast carcinoma tissue (Pietersen et al., 2008)
Biological Information
ImmunogenMBP-tagged recombinant protein corresponding to human EZH2.
CloneAE25
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ENX-1
  • KMT6
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~80 kDa observed. Uniprot describes multiple isoforms ranging from 80-86 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in T47D cell lysate.

Western Blot Analysis: A 1:1000 dilution of this antibody detected EZH2 in 10 µg of T47D cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE362 04053252551819

Documentation

Anti-EZH2 Antibody, clone AE25 SDS

Title

Safety Data Sheet (SDS) 

Anti-EZH2 Antibody, clone AE25 Certificates of Analysis

TitleLot Number
Anti-EZH2, clone AE25 - 2458016 2458016
Anti-EZH2, clone AE25 - 3472441 3472441
Anti-EZH2, clone AE25 - 3698189 3698189
Anti-EZH2, clone AE25 - 4154506 4154506
Anti-EZH2, clone AE25 - Q2062626 Q2062626
Anti-EZH2, clone AE25 -2572523 2572523
Anti-EZH2, clone AE25 -2621117 2621117
Anti-EZH2, clone AE25 Monoclonal Antibody 3048308
Anti-EZH2, clone AE25 Monoclonal Antibody 2902715

References

Reference overviewPub Med ID
EZH2 and BMI1 inversely correlate with prognosis and TP53 mutation in breast cancer.
Pietersen, Alexandra M, et al.
Breast Cancer Res., 10: R109 (2008)  2008

Show Abstract 		19099573 19099573