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MAB2626 Anti-Endosialin Antibody, clone B1/35

Detect Endosialin using this Anti-Endosialin Antibody, clone B1/35 validated for use in WB, IP, IF, FC.

Anti-Endosialin Antibody, clone B1/35 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: CD248, TEM1, CD164L1 UniProt Number: Q9HCU0
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MAB2626
200 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, IF, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2626
DescriptionAnti-Endosialin Antibody, clone B1/35
Alternate Names
  • CD248 molecule, endosialin
  • CD164 sialomucin-like 1
  • endosialin
  • CD248 antigen, endosialin
  • Tumor endothelial marker 1
  • CD248 antigen
Background InformationEndosialin is a glycoprotein on the cell surface of vascular endothelial cells in a broad range of human cancers. Endosialin is thought to be highly expressed at the points of active tissue remodeling and neovascularisation which may imply that endosialin is key in tumor growth, angiogenesis and metastasis. Endosialin has structural similarity to CD93, which involves a C-type lectin-like domain, EGF-like repeats and a glycosylated mucin-like domain. This similarity may also suggest that endosialin plays a role in innate immunity and inflammation.
References
Product Information
FormatPurified
Control
  • AG1523 cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Endosialin using this Anti-Endosialin Antibody, clone B1/35 validated for use in WB, IP, IF, FC.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunofluorescence
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A previous lot was used by an independent laboratory in FC to detect Endosialin in rheumatoid fibroblasts (MacFadyen, J. et al. (2005). FEBS. 579(12):2569-2575).

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP on AG1523 human fibroblasts (MacFadyen, J. et al. (2005). FEBS. 579(12):2569-2575).

Immunofluorescent Analysis: A previous lot was used by an independent laboratory in IF to detect Endosialin in human rheumatoid synovium, tonsil, salivary gland and normal breast tissues (MacFadyen, J. et al. (2005). FEBS. 579(12):2569-2575).
Biological Information
ImmunogenDiploid human fibroblasts
CloneB1/35
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD248
  • TEM1
  • CD164L1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: May play a role in tumor angiogenesis.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Probable.

TISSUE SPECIFICTY: Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.

PTM: O-glycosylated with sialylated oligosaccharides.

May be N-glycosylated.

SEQUENCE SIMILARITIES: Contains 1 C-type lectin domain.

Contains 1 EGF-like domain.

Contains 1 Sushi (CCP/SCR) domain.
Molecular Weight~175 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in AG1523 cell lysate.

Western Blot Analysis: A 1:2,500 dilution of this antibody detected Endosialin in 10 µg of AG1523 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB2626 04053252482458

Documentation

Anti-Endosialin Antibody, clone B1/35 SDS

Title

Safety Data Sheet (SDS) 

Anti-Endosialin Antibody, clone B1/35 Certificates of Analysis

TitleLot Number
Anti-Endosialin, clone B1/35 2475034
Anti-Endosialin, clone B1/35 - 1953015 1953015
Anti-Endosialin, clone B1/35 - 1987097 1987097
Anti-Endosialin, clone B1/35 - 2009453 2009453
Anti-Endosialin, clone B1/35 - 2043215 2043215
Anti-Endosialin, clone B1/35 - 2049281 2049281
Anti-Endosialin, clone B1/35 - 2211068 2211068
Anti-Endosialin, clone B1/35 - 3388679 3388679
Anti-Endosialin, clone B1/35 - 3862690 3862690
Anti-Endosialin, clone B1/35 - 3900078 3900078
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