Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABE1898-25UL Anti-FUS (TLS) Antibody, Clone 10F7

Anti-FUS (TLS), Clone 10F7, Cat. No. MABE1898, is a mouse monoclonal antibody that detects RNA-binding protein FUS and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

MABE1898-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABE1898-25UL
DescriptionAnti-FUS (TLS) Antibody, Clone 10F7
Alternate Names
  • RNA-binding protein FUS
  • 75 kDa DNA-pairing protein
  • Oncogene FUS
  • Oncogene TLS
  • POMp75
  • Translocated in liposarcoma protein
Background InformationRNA-binding protein FUS (UniProt: P35637; also known as 75 kDa DNA-pairing protein, Oncogene FUS, Oncogene TLS, POMp75, Translocated in liposarcoma protein) is encoded by the FUS (also known as TLS) gene (Gene ID: 2521) in human. Fused in sarcoma/translocated in liposarcoma FUS/TLS is a multi-functional nuclear protein that binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in super helical double-stranded DNA. It plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. It may also play a role in maintenance of genomic integrity. It binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. FUS is reported to be involved in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair. A chromosomal aberration involving FUS is shown to cause of acute myeloid leukemia (AML). Mutations in FUS gene have been linked to Amyotrophic lateral sclerosis 6, with or without frontotemporal dementia that affects upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. (Ref.: Lagier-Tourenne, C., et al. (2010). Hum. Mol. Genet. 19(1); R46-R64).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-FUS (TLS), Clone 10F7, Cat. No. MABE1898, is a mouse monoclonal antibody that detects RNA-binding protein FUS and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected FUS (TLS) in HeLa cell lysate.

Western Blotting Analysis: A representative lot detected FUS (TLS) in Western Blotting applications (Zhou, Y., et. al. (2013). PLoS Genet. 9(10):e1003895).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected FUS (TLS) in Chromatin Immunoprecipitation applications (Zhou, Y., et. al. (2013). PLoS Genet. 9(10):e1003895).

Immunoprecipitation Analysis: A representative lot detected FUS (TLS) in Immunoprecipitation applications (Zhou, Y., et. al. (2013). PLoS Genet. 9(10):e1003895).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged full-length recombinant human FUS protein.
Clone10F7
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 10F7 is a mouse monoclonal antibody that detects RNA binding protein FUS.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FUS
  • TLS
Purification MethodProtein G purified
UniProt Number
Molecular Weight~65 kDa observed; 53.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FUS (TLS) in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1898-25UL 04061842196272