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MAB3061 Anti-Fas Antibody, clone SM1/1

This Anti-Fas Antibody, clone SM1/1 is validated for use in FC, FUNC for the detection of Fas.

Anti-Fas Antibody, clone SM1/1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: FAS, APT1, CD95, FASTM, NMP35, FAS1, APO-1, ALPS1A, FAIM2, OTTHUMP00000059646, TNFRSF6, LFG UniProt Number: P25445
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MAB3061
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3061
ReplacesCBL527B
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Fas Antibody, clone SM1/1
Alternate Names
  • CD95
  • Apo-1
References
Product Information
FormatPurified
PresentationPurified IgG provided in PBS, no preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-Fas Antibody, clone SM1/1 is validated for use in FC, FUNC for the detection of Fas.
Key Applications
  • Flow Cytometry
  • Affects Function
Application NotesFlow cytometry: 1-10 μg/mL, with incubation for 30 minutes at 4°C. Daudi or L929 cells may be used as negative controls.

Induction of apoptosis with SM1/1:

SM1/1 induces apoptosis best when used together with a 10 molar excess of crosslinking anti-mouse IgG. The amount of antibody needed will vary depending upon the cell line used and the age of the cell and their growing conditions; best results are achieved when cells are less than 70% confluent and relatively young passage numbers, and note not all cells what express CD95 can be induced with SM1/1, the reasons why are unclear.

On Jurkat cells 100-500ng/mL of SM1/1 in the presence of 10X excess of goat anti-mouse IgG will produce ~50% kill as measured by MTT assay; without crosslinking, little or no killing will be observed.

On SKw6.4 cells 100-500ng/mL of SM1/1 in the presence of 10X excess goat anti-mouse IgG will produce ~50% kill as measured by MTT; without crosslinking ~20% or less of the cells will be induced.

On L/F15 cells 100-500ng/mL of SM1/1 with or without crosslinking IgG ~ 50% of the cells will be induced to undergo apoptosis as measured by MTT assay.

In all cases it is important to remove excess SM1/1 before adding the secondary crosslinking antibody;

Antibody additions can be either at 4C, or 37C; at 37C primary antibody should be incubated no longer than two hours prior to secondary crosslinker addition. Crosslinking antibody is typically incubated overnight, although shorter times may yield acceptable results.

As controls, cells should be incubated with an unrelated irrelevant isotype matched IgG2a antibody or with the crosslinking antibody alone.

SM1/1 monoclonal characterization is first reported in Trauth, BC et al Science 1989 245:301-5.



Optimal working dilutions must be determined by end user.
Biological Information
CloneSM1/1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificitySpecifically recognizes Fas [CD95/APO-1]
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
Gene Symbol
  • FAS
  • APT1
  • CD95
  • FASTM
  • NMP35
  • FAS1
  • APO-1
  • ALPS1A
  • FAIM2
  • OTTHUMP00000059646
  • TNFRSF6
  • LFG
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P25445 # Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
SIZE: 335 amino acids; 37732 Da
SUBUNIT: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE.
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted. & Isoform 6: Secreted.
TISSUE SPECIFICITY: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
DOMAIN: SwissProt: P25445 Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
DISEASE: SwissProt: P25445 # Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
SIMILARITY: Contains 1 death domain. & Contains 3 TNFR-Cys repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots. After opening, aliquot and freeze at -20°C. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB3061 04053252663857

Documentation

Anti-Fas Antibody, clone SM1/1 SDS

Title

Safety Data Sheet (SDS) 

Anti-Fas Antibody, clone SM1/1 Certificates of Analysis

TitleLot Number
Anti-Fas, clone SM1/1 - 1955313 1955313
Anti-Fas, clone SM1/1 - 3864326 3864326
Anti-Fas, clone SM1/1 - 4197627 4197627
Anti-Fas, clone SM1/1 - NG1871315 NG1871315
Anti-Fas, clone SM1/1 Monoclonal Antibody 3142457

References

Reference overviewPub Med ID
Inhibition of keratinocyte apoptosis by IL-15: a new parameter in the pathogenesis of psoriasis?
Ruckert, R, et al
J Immunol, 165:2240-50 (2000)  2000

10925312 10925312
Monoclonal antibody mediated tumor regression by induction of apoptosis {original article}
Trauth, B et al
Science, 245:301-305 (1989)  1989

2787530 2787530