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06-725-I Anti-FcεRI α subunit Antibody

Detect FcεRI α subunit using this Anti-FcεRI α subunit Antibody validated for use in Western Blotting, Immunoprecipitation, ICC.

Anti-FcεRI α subunit Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: FCER1A, FCE1A UniProt Number: P12319
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06-725-I
200 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, ICCRbSerumPolyclonal Antibody
Description
Catalogue Number06-725-I
Replaces06-725
DescriptionAnti-FcεRI α subunit Antibody
Alternate Names
  • High affinity immunoglobulin epsilon receptor subunit alpha
  • Fc-epsilon RI-alpha
  • FcERI
  • IgE Fc receptor subunit alpha
Background InformationThe allergic reaction is mediated through the IgE high affinity receptor (FcεRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FcεRI receptor complexes causes the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FcεRI receptor is a tetrameric complex, consisting of an a-chain, b-chain and a dimeric g-chain. The a-chain is primarily involved with the binding of IgE, but the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FcεRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.
References
Product Information
FormatSerum
Control
  • RBL cell lysate
PresentationRabbit polyclonal serum containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect FcεRI α subunit using this Anti-FcεRI α subunit Antibody validated for use in Western Blotting, Immunoprecipitation, ICC.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesWestern Blot Analysis: A 1:5,000 dilution from a representative lot detected FcεRI α subunit in 10 µg of human spleen tissue lysate.

Immunoprecipitation Analysis: A 1:50 dilution from a representative lot immunoprecipitated FcεRI α subunit from human spleen whole lysate.

Flow Cytometry Analysis: A 1:200 dilution from a representative lot detected FcεRI α subunit in RBL cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the extracellular domain of human FcεRI α subunit.
EpitopeExtracellular domain
HostRabbit
SpecificityThis antibody recognizes the extracellular domain of FcεRI α subunit.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FCER1A
  • FCE1A
Purification MethodUnpurified
UniProt Number
Molecular Weight~57 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in RBL cell lysate.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected FcεRI α subunit in 10 µg of RBL cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
06-725-I 04053252697272