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MAB1680-C Anti-Filamin A Antibody, clone TI10, Ascites Free

This Anti-Filamin A Antibody, clone TI10, Ascites Free is validated for use in western blotting, IHC, IP, flow cytometry, immunofluorescence & ICC for the detection of Filamin A.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: FLNA, FNL, FLN1 UniProt Number: P21333
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MAB1680-C
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, IP, FC, IF, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1680-C
DescriptionAnti-Filamin A Antibody, clone TI10, Ascites Free
Alternate Names
  • Filamin-A
  • FLN-A
  • Actin-binding protein 280
  • ABP-280
  • Alpha-filamin
  • Endothelial actin-binding protein
  • Filamin-1
  • Non-muscle filamin
Background InformationFilamin is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin binding site ath the other. It is present in smooth muscle, fibroblasts, platelets and lymphocytes.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Filamin A Antibody, clone TI10, Ascites Free is validated for use in western blotting, IHC, IP, flow cytometry, immunofluorescence & ICC for the detection of Filamin A.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunoprecipitation
  • Flow Cytometry
  • Immunofluorescence
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A 1:50 and 1:500 dilution from a representative lot detected Filamin A in human prostate and kidney tissues, respectively.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Filamin A in human tumor tissue sections (Bedolla, R. G., et al. (2009). Clin Cancer Research. 15(3):788-796.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in human normal and tumor tissue lysates (Bedolla, R. G., et al. (2009). Clin Cancer Research. 15(3):788-796.).

Immunoprecipitation/Western Blotting Analysis: A representative lot from an independent laboratory immunoprecipitated Filamin A from transfected HEK293T cell lysates. The immunoprecipitated sample was then subjected to Western Blotting using the same lot of antibody (Nakagawa, K., et al. (2010). Biochem. J. 427(2):237–245.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in transfected U937 cell lysate (Beekman, J. M., et al. (2008). J Immunol. 180(6):3938-3945.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in prostate cancer cell lysate. (Lin, J., et al. (2007). Int. J. Cancer. 121(12):2596–2605.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in LNCap and C4-2 whole cell lysates (Wang, Y., et al. (2007). Oncogene. 26(41):6061–6070.).

Western Blotting Analysis: A representative lot from an independent laboratory detected pulled-down human Filamin A proteins. (Lad, Y., et al. (2007). EMBO J. 26(17):3993–4004.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in lysate samples from normal bladder mucosa and muscle invasive bladder tumor (Smith, S. C., et al. (2007). Clin Cancer Res. 13(13):3803-3813.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Filamin A in lysate samples from human prefontal cortical tissue (Koh, P. O., et al. (2003). Ach Gen Psychiatry. 60(3):311-319.).

Flow Cytometry Analysis: A representative lot from an independent laboratory detected Filamin A in transfected U937 cells (Beekman, J. M., et al. (2008). J Immunol. 180(6):3938-3945.).

Flow Cytometry Analysis: A representative lot from an independent laboratory detected Filamin A in LAN-1 cells (Bachmann, A. S., et al. (2006) Cancer Sci. 97(12):1359–1365.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Filamin A in clinical patient samples (Kley, R. A., et al. (2007). Brain. 130(Pt 12):3250-3264.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Filamin A in LAN-1 cells (Bachmann, A. S., et al. (2006) Cancer Sci. 97(12):1359–1365.).
Biological Information
ImmunogenHuman platelet protein corresponding to human Filamin A.
CloneTI10
Concentration1 mg/mL
HostMouse
SpecificityThis antibody recognizes Filamin A (actin-binding protein), both the unprocessed form (270-280 kDa), and the C-terminal (90-100 kDa) calpain cleavage fragment of Filamin A (Aakhus, 1992).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FLNA
  • FNL
  • FLN1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~110 kDa observed. Phosphorylation of this protein may cause the band to shift in Western Blotting.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human uterus tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Filamin A in 10 µg of human uterus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB1680-C 04053252972614

Documentation

Anti-Filamin A Antibody, clone TI10, Ascites Free Certificates of Analysis

TitleLot Number
Anti-Filamin A, clone TI10, Ascites Free - Q2420920 Q2420920
Anti-Filamin A, clone TI10, -2679494 2679494
Anti-Filamin A, clone TI10, -2687826 2687826
Anti-Filamin A, clone TI10, -2722980 2722980
Anti-Filamin A, clone TI10, -2821246 2821246
Anti-Filamin A, clone TI10, Ascites Free - 3275529 3275529
Anti-Filamin A, clone TI10, Ascites Free - 3517952 3517952
Anti-Filamin A, clone TI10, Ascites Free - 3690685 3690685
Anti-Filamin A, clone TI10, Ascites Free - 4139222 4139222
Anti-Filamin A, clone TI10, Ascites Free Monoclonal Antibody 3012265