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MABF2659-100UG Anti-Flagellar Membrane Glycoprotein 1B (FMG-1B) Antibody, clone 61

Anti-Flagellar Membrane Glycoprotein 1B (FMG-1B), clone 61, Cat. No. MABF2659, is mouse monoclonal antibody that detects FMG-1B and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

MABF2659-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2659-100UG
DescriptionAnti-Flagellar Membrane Glycoprotein 1B (FMG-1B) Antibody, clone 61
Alternate Names
  • FMG-1B
Background InformationFlagella membrane glycoprotein 1B (UniProt: Q84X68; also known as FMG-1B) is derived from the flagella of single cell green algae Chlamydomonas reinhardtii. FMG-1B is a major membrane glycoprotein in the flagella of single green alga, Chlamydomonas. It is exposed at the flagellar surface and is reported to be the only flagellar protein in contact with the substrate during whole cell gliding motility and microsphere movement. It contains an N-terminal signal sequence, a transmembrane domain (aa 437-4160), and a short cytoplasmic tail of 17 amino acids that extends into the flagellar matrix. Chlamydomonas are reported to exhibit four types of flagella-dependent motilities described as: forward swimming where flagella leads the cell body, a reverse direction swim using a symmetrical sine wave pattern, flagellar surface interaction with substrate to facilitate whole cell gliding, and the intraflagellar transport (IFT) which moves multi-subunit protein complexes from the flagellar base to the tip in an antegrade direction. The anterograde IFT is reported to be powered by the activity of kinesin 2. Although FMG-1B is shown to be distributed along the surface of the flagellum, it also serves as a cargo for IFT machinery.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Flagellar Membrane Glycoprotein 1B (FMG-1B), clone 61, Cat. No. MABF2659, is mouse monoclonal antibody that detects FMG-1B and is tested for use in ELISA, Immunofluorescence, and Western Blotting.
Key Applications
  • ELISA
  • Immunofluorescence
  • Western Blotting
Application NotesImmunofluorescence Analysis: A 1:250 dilution from a representative lot detected Flagellar Membrane Glycoprotein 1B (FMG-1B) in gluteraldehyde fixed Chlamydomonas reinhardtii.

ELISA Analysis: A representative lot detected Flagellar Membrane Glycoprotein 1B (FMG-1B) in ELISA applications (Bloodgood, RA., et. al. (1986). J Cell Biol. 102(5):1797-812).

Western Blotting Analysis: A representative lot detected Flagellar Membrane Glycoprotein 1B (FMG-1B) in Western Blotting applications (Bloodgood, R.A., et. al. (1986). J Cell Biol. 102(5):1797-812; Bloodgood, RA., et. al. (2019). J Cell Sci. 132(16):jcs233429).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified flagellar protein from Chlamydomonas reinhardtii.
EpitopeUnknown
Clone61
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 61 is a mouse monoclonal antibody that detects Flagellar Membrane Glycoprotein 1B (FMG-1B) in Chlamydomonas reinhardtii.
IsotypeIgG1κ
Species Reactivity
  • A Broad Range Of Species
Species Reactivity NoteChlamydomonas reinhardtii (Green Algae)
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Molecular Weight412.65 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF2659-100UG 04065265324168