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MAB10216-I-25UG Anti-Flavivirus Group Antigen Antibody, clone D1-4G2-4-15

Anti-Flavivirus Group Antigen, clone D1-4G2-4-15, Cat. No. MAB10216-I, is a mouse monoclonal antibody that detects Flavivirus Group Antigen and is tested for use in Immunofluorescence.

MAB10216-I-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAB10216-I-25UG
DescriptionAnti-Flavivirus Group Antigen Antibody, clone D1-4G2-4-15
Alternate Names
  • Flavivirus antigen
Background InformationThe family Flaviviridae includes a wide variety of enveloped viruses that infect both arthropods and vertebrates. Flaviviruses are positive, single-stranded enveloped RNA viruses. They are found mainly in ticks and mosquitoes and can occasionally infect humans. Flaviviruses contain a positive-sense single-stranded RNA genome (10.7 kb) that is transcribed into a single polyprotein precursor that encodes three structural proteins, the capsid (C), pre-membrane (prM), and envelope (E) proteins, and seven nonstructural proteins. The flavivirus E glycoprotein is shown to be essential for membrane fusion and binding to cellular receptors. Flaviviruses caused diseases include Yellow Fever, Dengue Fever, Japanese encephalitis, West Nile viruses, Zika virus, and are also responsible for encephalitis and hemorrhagic diseases. Clone D1-4G2-4-15 binds to flavivirus group antigen and targets an epitope in the fusion loop at the extremity of domain II of E protein. This epitope is shown to be highly conserved amongst Flaviviridae family members. This clone is sensitive to beta-mercaptoethanol treatment and loses its binding to all four E proteins under reducing conditions. (Ref.: Lai, C.Y., et al., (2008). J. Virol. 82(13); 6631-6643; Crill, WD., and Chang, GJJ (2004). J. Virol. 78(24); 13975-13986).
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Flavivirus Group Antigen, clone D1-4G2-4-15, Cat. No. MAB10216-I, is a mouse monoclonal antibody that detects Flavivirus Group Antigen and is tested for use in Immunofluorescence.
Key Applications
  • Immunofluorescence
Application NotesImmunofluorescence Analysis: A representative lot detected Flavivirus Group Antigen in Immunofluorescence applications (Haridas, V., et. al. (2013). PLoS Negl Trop Dis. 7(1):e2005).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenDengue virus type 2 antigens.
CloneD1-4G2-4-15
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone D1-4G2-4-15 is a mouse monoclonal antibody that detects Flavivirus Group Antigen. It targets an epitope within the fusion loop at the extremity of domain II of protein E.
IsotypeIgG2a
Species Reactivity
  • Virus
Species Reactivity NoteVirus
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
Molecular Weight55-70 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.



Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB10216-I-25UG 04061842196296

Documentation

Anti-Flavivirus Group Antigen Antibody, clone D1-4G2-4-15 SDS

Title

Safety Data Sheet (SDS) 

Anti-Flavivirus Group Antigen Antibody, clone D1-4G2-4-15 Certificates of Analysis

TitleLot Number
Anti-Flavivirus Group Antigen, clone D1-4G2-4-15 - 3827435 3827435
Anti-Flavivirus Group Antigen, clone D1-4G2-4-15 - 3837034 3837034
Anti-Flavivirus Group Antigen, clone D1-4G2-4-15 - 3862785 3862785
Anti-Flavivirus Group Antigen, clone D1-4G2-4-15 - 3868421 3868421
Anti-Flavivirus Group Antigen, clone D1-4G2-4-15 - 3917377 3917377
Anti-Flavivirus Group Antigen, clone D1-4G2-4-15 - 4046284 4046284
Anti-Flavivirus Group Antigen, clone D1-4G2-4-15 - 4052769 4052769
Anti-Flavivirus Group Antigen, clone D1-4G2-4-15 - 4055481 4055481
Anti-Flavivirus Group Antigen, clone D1-4G2-4-15 - 4057518 4057518
Anti-Flavivirus Group Antigen, clone D1-4G2-4-15 - 4083768 4083768
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References

Reference overviewPub Med ID
Bispidine-amino acid conjugates act as a novel scaffold for the design of antivirals that block Japanese encephalitis virus replication.
Haridas V, Rajgokul KS, Sadanandan S, Agrawal T, Sharvani V, Gopalakrishna MV, Bijesh MB, Kumawat KL, Basu A, Medigeshi GR.
PLoS Negl Trop Dis  7(1)  e2005  2013

Show Abstract 		23350007 23350007