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MABE985 Anti-Fox1 Antibody, clone 1D10

Anti-Fox1 Antibody, clone 1D10, Cat. No. MABE985, is a highly specific mouse Monoclonal antibody, that targets RNA binding protein fox-1 homolog 1 and has been tested in Western Blotting, Immunocytochemistry, Immunohistochemistry and RNA-Binding Immunoprecipitation/iCLIP.

Anti-Fox1 Antibody, clone 1D10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: Rbfox1, A2bp, A2bp1, Fox1, MNCb-3035 UniProt Number: Q9JJ43
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MABE985
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, IHC, ICC, RIPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE985
DescriptionAnti-Fox1 Antibody, clone 1D10
Alternate Names
  • RNA binding protein fox-1 homolog 1
  • Ataxin-2-binding protein 1
  • Fox-1 homolog A
  • Fox1
Background InformationRNA binding protein fox-1 homolog 1 (UniProt Q9JJ43; also known as Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide binding protein 1) is encoded by the Rbfox1 (also known as A2bp, A2bp1, Fox-1, Hrnbp1) gene (ORF MNCb-3035; Gene ID 268859) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Rbfox1 transcript itself is subjected to alternative splicing in response to chronic cell depolarization. This results in the increased expression of the nuclear, splicing-active Rbfox1 isoform, which in turn leads to increased splicing of Rbfox1 target transcripts of proteins, including N-methyl D-aspartate (NMDA) receptor 1 (Grin1) and a calcium ATPase (Atp2b1), that play important roles in neuronal excitation and calcium homeostasis.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Fox1 Antibody, clone 1D10, Cat. No. MABE985, is a highly specific mouse Monoclonal antibody, that targets RNA binding protein fox-1 homolog 1 and has been tested in Western Blotting, Immunocytochemistry, Immunohistochemistry and RNA-Binding Immunoprecipitation/iCLIP.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
  • RNA Binding Protein Immunoprecipitation (RIP)
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Fox1 in 10 µg of adult mouse frontal cortex tissue lysate.
Immunohistochemistry Analysis: A representative lot detected Fox1 in coronal sections from E18 mouse (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765).
Immunocytochemistry Analysis: A representative lot detected Fox1 in post-mitotic neuronal cells differentiated from mouse embryonal carcinoma P19 cells (Lee, J.A., et al. (2009). Genes Dev. 23(19):2284-2293).
RNA-Binding Protein Immunoprecipitation/iCLIP Analysis: A representative lot was employed to immunoprecipitate Fox1-associated pre-mRNAs from UV-crosslinked murine cortex homogenates by individual-nucleotide resolution crosslinking immunoprecipitation (iCLIP) for characterization of Fox1 pre-mRNA-binding sites (Gehman, L.T., et al. (2011) Nat Genet. 43(7):706-711).
Western Blotting Analysis: A representative lot detected Fox1 in mouse cortical tissue homogenate (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to mouse Fox1.
Clone1D10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Rbfox1
  • A2bp
  • A2bp1
  • Fox1
  • MNCb-3035
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~43 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in adult mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fox1 in 10 µg of adult mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE985 04055977176391

Documentation

Anti-Fox1 Antibody, clone 1D10 SDS

Title

Safety Data Sheet (SDS) 

Anti-Fox1 Antibody, clone 1D10 Certificates of Analysis

TitleLot Number
Anti-Fox1, clone 1D10 - 3238013 3238013
Anti-Fox1, clone 1D10 - 3397945 3397945
Anti-Fox1, clone 1D10 - 3715081 3715081
Anti-Fox1, clone 1D10 - 3756526 3756526
Anti-Fox1, clone 1D10 - 3894959 3894959
Anti-Fox1, clone 1D10 - 3952248 3952248
Anti-Fox1, clone 1D10 - 4176607 4176607
Anti-Fox1, clone 1D10 -2821646 2821646
Anti-Fox1, clone 1D10 -2884404 2884404
Anti-Fox1, clone 1D10 -Q2581260 Q2581260
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Technical Info

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White Paper: Further considerations of antibody validation and usage.