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ABE74 Anti-FoxP4 Antibody

Use Anti-FoxP4 Antibody (Rabbit Polyclonal Antibody) validated in ICC, WB, IHC to detect FoxP4 also known as forkhead box P4, winged-helix repressor FOXP4.

Anti-FoxP4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: Foxp4, FKHLA, hFKHLA UniProt Number: Q9DBY0
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ABE74
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICC, WB, IHCRbSerumPolyclonal Antibody
Description
Catalogue NumberABE74
DescriptionAnti-FoxP4 Antibody
Alternate Names
  • forkhead box P4
  • fork head-related protein like A
  • Fork head-related protein-like A
  • winged-helix repressor FOXP4
Background InformationForkhead box protein P4 (FoxP4) is a multidomain transcriptional repressor. This protein is a member of the Fox family of proteins characterized by it unique winged-helix DNA binding motif. Members of this family are known for their ability to regulate both the cell fate and differentiation of several tissues. FoxP4 acts to repress expression by inteacting with DNA. For DNA binding to occur, FoxP4 must form homodimers and heterodimers with FoxP1 and FoxP2. During embryogenesis, expression of this protein is observed in lung and brain. In adults expression is observed in adult heart, lung, brain, liver, spleen, testis and kidney. Alternative splicing result in the expression of three known isoforms.
References
Product Information
FormatSerum
Control
  • Human brain cell lysate
PresentationUnpurified rabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-FoxP4 Antibody (Rabbit Polyclonal Antibody) validated in ICC, WB, IHC to detect FoxP4 also known as forkhead box P4, winged-helix repressor FOXP4.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected FoxP4 in HeLa cells.

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Lu, M.M., et al. (2002). Mechanisms of Development 119S:S197–S202.)
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to mouse FoxP4.
EpitopeUnknown
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes FoxP4.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Foxp4
  • FKHLA
  • hFKHLA
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: Transcriptional repressor that represses lung-specific expression.

SUBUNIT STRUCTURE: Forms homodimers and heterodimers with FOXP1 and FOXP2. Dimerization is required for DNA-binding.

SUBCELLULAR LOCATION: Nucleus Probable.

TISSUE SPECIFICITY: Expressed in the adult heart, brain, spleen lung, liver, kidney and testes.

DEVELOPMENTAL STAGE: Expressed predominantly in the lung and brain during embryogenesis. Expressed in the lung epithelium and the mesenchyme immediately adjacent to the epithelium from 10.5 dpc. At 9.5 dpc, expressed in the foregut endoderm but not in the heart. At 16.5 and 18.5 dpc, expressed in both the proximal and distal airway epithelium. Also expressed in the developing gut. In the hindgut, primarily expressed in epithelial cells of the intestine and stomach. Expressed in the brain in a dynamic pattern. At 14.5 dpc, expressed at high levels in the intermediate zone of the neopallial cortex with lower levels in the surrounding cells. By 16.5 dpc, no longer expressed in the intermediate zone, but still present in the surrounding cells of the neopallial cortex.

DOMAIN: The leucine-zipper is required for dimerization and transcriptional repression.

SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger.

Contains 1 fork-head DNA-binding domain.
Molecular Weight 86 and 79 kDa Observed. Three known isoforms due to alternative splicing.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human brain cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected FoxP4 on 10 µg of human brain cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE74 04053252517570