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MABS819 Anti-GAPDH (CT) Antibody, clone RM114

This Anti-GAPDH (CT) Antibody, clone RM114 is validated for use in Western Blotting, Immunocytochemistry for the detection of GAPDH (CT).

Anti-GAPDH (CT) Antibody, clone RM114 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: GAPDH, GAPD, CDABP0047 UniProt Number: P04406
MABS819
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS819
DescriptionAnti-GAPDH (CT) Antibody, clone RM114
Alternate Names
  • Glyceraldehyde-3-phosphate dehydrogenase
  • GAPDH
  • Peptidyl-cysteine S-nitrosylase GAPDH
  • GAPDH
Background InformationGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in moderately high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
References
Product Information
FormatPurified
PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Quality LevelMQ300
Applications
ApplicationThis Anti-GAPDH (CT) Antibody, clone RM114 is validated for use in Western Blotting, Immunocytochemistry for the detection of GAPDH (CT).
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected GAPDH (CT) in A431 cells.
Biological Information
ImmunogenA peptide corresponding to the C-terminus of GAPDH.
EpitopeC-terminus
CloneRM114
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • GAPDH
  • GAPD
  • CDABP0047
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~39 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected GAPDH (CT) in A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS819 04055977181531

Documentation

Anti-GAPDH (CT) Antibody, clone RM114 SDS

Title

Safety Data Sheet (SDS) 

Anti-GAPDH (CT) Antibody, clone RM114 Certificates of Analysis

TitleLot Number
Anti-GAPDH (CT), clone RM114 -VP1811300 VP1811300
Anti-GAPDH (CT), clone RM114 - VP1912273 VP1912273
Anti-GAPDH (CT), clone RM114 - VP2108264 VP2108264
Anti-GAPDH (CT), clone RM114 - VP2309201 VP2309201
Anti-GAPDH (CT), clone RM114 - VP2407247 VP2407247
Anti-GAPDH (CT), clone RM114 -QVP1503207 QVP1503207
Anti-GAPDH (CT), clone RM114 Monoclonal Antibody VP1706195
Anti-GAPDH (CT), clone RM114 Monoclonal Antibody VP1807279

Brochure

Title
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Technical Info

Title
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White Paper: Further considerations of antibody validation and usage.

Posters

Title
Post-Translational Modifications