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MABD167 Anti-GATA1 Antibody, clone 4F5

Anti-GATA1 Antibody, clone 4F5 is a highly specific mouse monoclonal antibody, that targets GATA & has been tested in western blotting, ICC & IHC.

Anti-GATA1 Antibody, clone 4F5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: GF1, GF-1, NFE1, XLTT, ERYF1, GATA-1, GATA1 UniProt Number: P15976
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MABD167
100 µL  
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Overview

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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IHCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABD167
ReplacesMABE470
DescriptionAnti-GATA1 Antibody, clone 4F5
Alternate Names
  • Erythroid transcription factor
  • Eryf1
  • GATA-binding factor 1
  • GATA-1
  • GF-1
  • NF-E1 DNA-binding protein
Background InformationGATA-1 (GATA1), or alternatively Gata-binding factor 1, GF-1, NF-E1 DNA binding protein, and Erythroid transcription factor (EryF1) encoded by the human gene named GATA1/ERYF1/GF1 is transcriptional activator central for erythroid development. GATA1 also plays critical roles in controlling gene expression in other system as well. Research has shown that GATA1 contributes to the level of alpha synuclein gene expression in Parkinson’s disease and over expression of GATA1 in cortical neurons leads to a decrease in the expression of Rab4b and reduces dendrite branching and maturation, and GATA1 expression is elevated in brains with depressive disorder suggesting that GATA1 may be new target for anti-depressant therapies. EMD-Millipore’s Anti-GATA1 mouse monoclonal antibody has been tested in western blot on K562 cell lysates and paraffin embedded immunohistochemistry on pancreatic cancer tissues and in fluorescent immunocytochemistry on K562 cells in culture.
References
Product Information
FormatAscites
Control
  • K562 cell lysate
PresentationMouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GATA1 Antibody, clone 4F5 is a highly specific mouse monoclonal antibody, that targets GATA & has been tested in western blotting, ICC & IHC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GATA1 in K562 cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA1 in pancreatic cancer tissue.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified recombinant fragment of human GATA1 expressed in E. Coli.
Clone4F5
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • GF1
  • GF-1
  • NFE1
  • XLTT
  • ERYF1
  • GATA-1
  • GATA1
Purification MethodUnpurified
UniProt Number
Molecular Weight~45 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GATA1 in K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABD167 04053252918100

Documentation

Anti-GATA1 Antibody, clone 4F5 SDS

Title

Safety Data Sheet (SDS) 

Anti-GATA1 Antibody, clone 4F5 Certificates of Analysis

TitleLot Number
Anti-GATA1, clone 4F5 - QVP1305204 QVP1305204
Anti-GATA1, clone 4F5 - VP2001070 VP2001070
Anti-GATA1, clone 4F5 -VP1410070 VP1410070
Anti-GATA1, clone 4F5 -VP1505073 VP1505073
Anti-GATA1, clone 4F5 -VP1507159 VP1507159
Anti-GATA1, clone 4F5 -VP1509243 VP1509243
Anti-GATA1, clone 4F5 -VP1512152 VP1512152
Anti-GATA1, clone 4F5 -VP1607213 VP1607213
Anti-GATA1, clone 4F5 Monoclonal Antibody VP1705255