Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

ABN993 Anti-GHRH (Somatoliberin)

Anti-GHRH (Somatoliberin) Antibody, Cat. No. ABN993, is a highly specific sheep polyclonal antibody that targets Somatoliberin (GHRH) and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Radioimmunoassay.

Anti-GHRH (Somatoliberin) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Ghrh UniProt Number: P09916
View Products on Sigmaaldrich.com
ABN993
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, MIF, IH(P), RIAShUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABN993
DescriptionAnti-GHRH (Somatoliberin)
Alternate Names
  • Somatoliberin
  • Growth hormone-releasing factor
  • GRF
  • Growth hormone-releasing hormone
Background InformationSomatoliberin (UniProt: P09916; also known as Growth hormone-releasing factor, GRF, Growth hormone-releasing hormone, GHRH) is encoded by the Ghrh gene (Gene ID: 29446) in rat. GHRH F is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone. The release of growth hormone from pituitary gland depends of the interplay between the stimulatory action of GHRH and inhibitory action of somatotropin release inhibiting factor (SRiF) or somatostatin. These two neurohormones are shown to be released in a reciprocal manner from the median eminence into the portal circulation and result in ultradian rhythmic release pattern of growth hormone.. Post-translational removal of the signal peptide (aa 1-19) and the two propeptide sequences (aa 20-30) and aa 75-104) releases the 43 aa mature Somatoliberin (aa 31-73), which functions as a ligand to stimulate growth hormone (GH) release from the pituitary. Variant receptors for GHRH have been found in several types of tumors, and antagonists of these receptors are shown to block the growth of the tumors. Defects in Ghrh gene are a known to cause of dwarfism, while hypersecretion of the encoded GHRH can lead to gigantism.
References
Product Information
FormatUnpurified
PresentationSheep polyclonal antiserum with 0.01% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GHRH (Somatoliberin) Antibody, Cat. No. ABN993, is a highly specific sheep polyclonal antibody that targets Somatoliberin (GHRH) and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Radioimmunoassay.
Key Applications
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Radioimmunoassay
Application NotesRadioimmunoassay Analysis: A representative lot detected GHRH (Somatoliberin) in Radioimmunoassay applications (Tannenbaum, G.S., et. al. (1990). Endocrinology. 127(6):3087-95).

Immunohistochemistry Analysis: A representative lot detected GHRH (Somatoliberin) in Immunohistochemistry applications (Tannenbaum, G.S., et. al. (1990). Endocrinology. 127(5):2551-60; Epelbaum, J., et. al. (1989). J Neuroendocrinol. 1(2):109-15).

Immunofluorescence Analysis: A representative lot detected GHRH (Somatoliberin) in Immunofluorescence applications (Stroh, T., et. al. (2009). J Neurosci. 29(25):8198-205).
Biological Information
ImmunogenBSA-conjugated synthetic linear peptide corresponding to the first 29 amino acids from rat Somatoliberin (GHRH).
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostSheep
SpecificityThis sheep polyclonal antibody detects somatolierin (GHRH) in mouse and rat brain sections. It targets an epitope within the first 29 amino acids.
IsotypeIgG
Species Reactivity
  • Rat
  • Mouse
Species Reactivity NoteMouse, Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ghrh
Purification MethodUnpurified
UniProt Number
Molecular Weight12.27 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse brain lateral hypothalamic region.

Immunohistochemistry Analysis: A 1:2,000 dilution of this antibody detected GHRH (Somatoliberin) in mouse brain lateral hypothalamic region.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABN993 04054839328831