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MABS132-25UL Anti-GLUT1 (C-terminus) Antibody, clone 5B12.3 (mouse monoclonal)

Anti-GLUT1 (C-terminus) Antibody, clone 5B12.3 is an antibody against GLUT1 (C-terminus) for use in Western Blotting & IHC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: SLC2A1, GLUT1 UniProt Number: P11166
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MABS132-25UL
25 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS132-25UL
DescriptionAnti-GLUT1 (C-terminus) Antibody, clone 5B12.3 (mouse monoclonal)
Alternate Names
  • Solute carrier family 2 facilitated glucose transporter member 1
  • Glucose transporter type 1,erythrocyte/brain
  • GLUT-1
  • HepG2 glucose transporter
Background InformationGLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55 kDa and a 45 kDa. The 55 kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake.
References
Product Information
FormatPurified
Control
  • NGF treated PC-12 cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-GLUT1 (C-terminus) Antibody, clone 5B12.3 is an antibody against GLUT1 (C-terminus) for use in Western Blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLUT1 in rat cerebellum and human cerebellum tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human GLUT1.
EpitopeC-terminus
Clone5B12.3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThis antibody recognizes GLUT1 at the C-terminus.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC2A1
  • GLUT1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~54 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in NGF treated PC-12 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected GLUT1 in 10 µg of NGF treated PC-12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS132-25UL 04054839350504